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Phospho-Akt1 (Ser473) Fluorometric Cell-Based ELISA Kit
Phospho-Akt1 (Ser473) Fluorometric Cell-Based ELISA Kit

Assay Biotechnology Inc Antibodies and ELISA Kits have been rigorously validated for laboratory grade quality results. High specificity paired with high affinity for targets of interest give researchers an advantage with our products

Catalog No: FLUO-CBP1490
Reactivity:H:S473,M:S473,R:S473
Assay Type:Phospho Cell-Based ELISA
Format:2 Plates: TC 96-Well Plates
Dynamic Range:At least 5000 cells/well

$500
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Intra-well Normalization
GAPDH Actin or beta Catenin controls
Ex/Em: 651/667 - 495/521
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Phospho-Akt1 (Ser473) Fluorometric Cell-Based ELISA Kit

Western blot analysis of extracts from HeLa cells treated with heat shock, using Akt (Phospho-Ser473) Antibody.

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Product Details
Product Name
Phospho-Akt1 (Ser473) Fluorometric Cell-Based ELISA Kit
Description
The Phospho-Akt1 (Ser473) Fluorometric Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor Phospho-Akt1 (Ser473) protein phosphorylation and expression profile in cells. The kit can be used for measuring the relative amounts of phosphorylated Phospho-Akt1 (Ser473) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals, or activators have on CAV1 phosphorylation.
Catalog No
FLUO-CBP1490
Format
2 Plates: TC 96-Well Plates
Detection Target
Akt1 (Phospho-Ser473)
Reactivity
H:S473, M:S473, R:S473
Detection Method
Fluorometric
Assay Type
Phospho Cell-Based ELISA
SwissProt ID
P31749
NCBI Gene ID
AKT1/AKT2/AKT3
Target Synonyms
AKT; AKT1 kinase; C-AKT; kinase Akt1; PKB; PKB-alpha; Protein kinase B; RAC; RAC-alpha serine/threonine kinase; RAC-PK-alpha
Storage and Stability Guarantee
4°C/6 Months
Sample Type
Researchers plate their cell line of choice
Range
At least 5000 cells/well
Assay Time
4.5 hours
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