The AMPKα1 (Phospho-Ser485) Colorimetric Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can detect AMPKα1 (Phospho-Ser485) protein expression profile in cells. The kit can be used for measuring the relative amounts of AMPKα1 (Phospho-Ser485) in cultured cells as well as screening for the effects that variuos treatments, inhibitors (ie siRNA or chemicals), or activators have on AMPKα1 (Phospho-Ser485).
This kit contains Crystal Violet Staining normalization protocol. It provides a means to normalize Absorbance 450 nm of primary antibodies to cell density of the cells in the same well. This provides an accurate account for stimulations and cell densities during the assay. By comparing the proportional values obtained from the experimental wells to those of the control wells (e.g. untreated cells), it is possible to determine the extent to which the stimulant(s) have affected the expression levels.
Western blot analysis of lysates from HeLa cells treated with heat shock, using AMPK1 (Phospho-Ser485) Antibody. The lane on the right is blocked with the phospho peptide.
