The Tfact Smad1 TFact™ DNA-Binding ELISA contains components necessary for detection and qualitative analysis of Smad1 active transcription factors in eukaryotic nuclear or cell lysates. Tfact ELISA kits are designed to significantly reduce experiment time and ensure sensitivity and flexibility for high-throughput screening.
The TFact™ Smad1 DNA-Binding ELISA detects active Smad1 in HepG2 and Jurkat Nuclear Extract. The HepG2 and Jurkat cells were grown 3 days in DMEM and RPMI 1640 with 10% FBS and harvested for nuclear extract.