The Tfact SP3/4 TFact™ DNA-Binding ELISA contains components necessary for detection and qualitative analysis of SP3/4 active transcription factors in eukaryotic nuclear or cell lysates. Tfact ELISA kits are designed to significantly reduce experiment time and ensure sensitivity and flexibility for high-throughput screening.
The TFact™ SP3/4 DNA-Binding ELISA detects active SP3/4 in Hela Nuclear Extract. The Hela cells were grown 3 days in DMEM with 10% FBS and harvested for nuclear extract. The Hela cells were stimulated by PMA (200nM) before harvest.
