The Tfact KLF TFact™ DNA-Binding ELISA contains components necessary for detection and qualitative analysis of KLF active transcription factors in eukaryotic nuclear or cell lysates. Tfact ELISA kits are designed to significantly reduce experiment time and ensure sensitivity and flexibility for high-throughput screening.
The TFact™ KLF DNA-Binding ELISA detects active KLF in HepG2 and MCF7 Nuclear Extract. The HepG2 and MCF7 cells were grown 3 days in DMEM with 10% FBS and harvested for nuclear extract.