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TNF-alpha (Murine) OmniKine™ ELISA Kit
TNF-alpha (Murine) OmniKine™ ELISA Kit

Assay Biotechnology Inc Antibodies and ELISA Kits have been rigorously validated for laboratory grade quality results. High specificity paired with high affinity for targets of interest give researchers an advantage with our products

Catalog No: OK-0200
Reactivity:Mouse
Assay Type:Sandwich ELISA
Format:1 plate: 12 x 8-Well Microstrips
Dynamic Range:16-2000 pg/mL

$310
# Datasheet #
# SDS #
Recovery and Linearity 80-120%
Sample Specific Diluent
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TNF-alpha (Murine) OmniKine™ ELISA Kit

The data and subsequent graph were obtained after performing a cytokine ELISA for TNF-alpha. Each known sample concentration was assayed in triplicate. The OmniKine™ Murine TNF-alpha ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Murine TNF-alpha proteins within the range of 16-2000 pg/ml.

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Product Details
Product Name
TNF-alpha (Murine) OmniKine™ ELISA Kit
Description
The TNF-alpha (Murine) OmniKine™ ELISA Kit contains the components necessary for quanititative determination of natural or recombinant TNF-alpha concentrations within any experimental sample including cell lysates, serum and plasma.
Catalog No
OK-0200
Format
1 plate: 12 x 8-Well Microstrips
Detection Target
TNF-alpha
Reactivity
Mouse
Recovery Data
Three concentrations of recombinant Murine TNF-alpha was spiked into various samples to measure the recovery percentage of recombinant protein in duplicate. The average recovery for Serum and RPMI FBS 10% were as follows: 93 and 84.
Linearity Data
Murine TNF-alpha recombinant protein was spiked into various biological samples and serial diluted with sample specific diluent for Serum and RPMI FBS10%. The average linearities for Serum and RPMI FBS10% were as follows: 102.75 and 85.
Detection Method
Colorimetric 450 nm
Assay Type
Sandwich ELISA
SwissProt ID
P06804
NCBI Gene ID
21926
Target Synonyms
Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNFSF2
Storage and Stability Guarantee
4°C/3 Months
Sample Type
Plasma, Serum, Cell Culture Supernatant
Range
16-2000 pg/mL
Assay Time
4.5 hours
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Primary Antibodies

    • ELISA Kits

    • Research Areas

    Cell Adhesion Proteins Cell Cycle Proteins Channel Proteins Exosome GDP/GTP Binding Proteins Growth Factors and Hormones Homeodomain Proteins Immunohistochemical Kinases and Phosphatases Signaling

     

    Membrane Receptors Neurobiology Signaling Intermediates Steroid Receptors Structural Proteins Synthesis and Degradation Transcription Regulators Transport and Trafficking Tumor Suppressors/Apoptosis
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