}
PI3-kinase p85-α/γ Colorimetric Cell-Based ELISA Kit
Catalog No :
CB5553
Reactivity
Human,Mouse,Rat
Assay Type
Cell-Based ELISA
Format
1 Plate: TC 96-Well Plate
Dynamic Range
At least 5000 cells/well
Size
Price/Quantity
$400
Quantity
Details
Product Name
PI3-kinase p85-α/γ Colorimetric Cell-Based ELISA Kit
Description
The PI3-kinase p85-α/γ Colorimetric Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can detect PI3-kinasep85-α/γ protein expression profile in cells. The kit can be used for measuring the relative amounts of PI3-kinasep85-α/γ in cultured cells as well as screening for the effects that variuos treatments, inhibitors (ie siRNA or chemicals), or activators have on PI3-kinasep85-α/γ.
Catalog No
CB5553
Format
1 Plate: TC 96-Well Plate
Detection Target
PI3-kinasep85-α/γ
Reactivity
Human, Mouse, Rat
Detection Method
Colorimetric 450 nm
Assay Type
Cell-Based ELISA
SwissProt ID
P27986/Q92569
NCBI Gene ID
5295/8503
Target Synonyms
GRB1; P85A; PI3-kinase p85-alpha subunit; PI3K; PI3K p85-alpha; Phosphatidylinositol 3-kinase regulatory alpha subunit; PtdIns-3-kinase p85-alpha; P55G; PI3-kinase p85-gamma subunit; PI3K p85-gamma; Phosphatidylinositol 3-kinase regulatory gamma subunit; PtdIns-3-kinase p85-gamma; p55PIK
Storage and Stability Guarantee
4°C/6 Months
Sample Type
Researchers plate their cell line of choice
Range
At least 5000 cells/well
Assay Time
4.5 hours
References:
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Abstract:
A short protocol of the steps taken in Assay Biotechnology's Cell-Based ELISA Kits.
An illustrated breakdown of the Colorimetric Cell-Based ELISA assay principles.
Western blot analysis of extracts from COS7 cells, treated with H2O2 100uM 30', using PI3-kinase p85-alpha/gamma (Ab-467/199) Antibody.
