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Phospho-PI3-kinase p85-α/γ (Tyr467/199) Fluorometric Cell-Based ELISA Kit
Phospho-PI3-kinase p85-α/γ (Tyr467/199) Fluorometric Cell-Based ELISA Kit
Catalog No :
FLUO-CBP1475
Reactivity
H:Y467/Y199,M:Y467/Y199,R:Y467/Y199
Assay Type
Phospho Cell-Based ELISA
Format
2 Plates: TC 96-Well Plates
Dynamic Range
At least 5000 cells/well
Size
Price/Quantity
$500
Quantity
Details
Product Name
Phospho-PI3-kinase p85-α/γ (Tyr467/199) Fluorometric Cell-Based ELISA Kit
Description
The Phospho-PI3-kinase p85-α/γ (Tyr467/199) Fluorometric Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor Phospho-PI3-kinase p85-α/γ (Tyr467/199) protein phosphorylation and expression profile in cells. The kit can be used for measuring the relative amounts of phosphorylated Phospho-PI3-kinase p85-α/γ (Tyr467/199) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals, or activators have on CAV1 phosphorylation.
Catalog No
FLUO-CBP1475
Format
2 Plates: TC 96-Well Plates
Detection Target
PI3-kinase p85-α/γ (Phospho-Tyr467/199)
Reactivity
H:Y467/Y199, M:Y467/Y199, R:Y467/Y199
Detection Method
Fluorometric
Assay Type
Phospho Cell-Based ELISA
SwissProt ID
P27986/Q92569
NCBI Gene ID
PIK3R1/PIK3R3
Target Synonyms
GRB1; P85A; PI3-kinase p85-alpha subunit; PI3K; PI3K p85-alpha; Phosphatidylinositol 3-kinase regulatory alpha subunit; PtdIns-3-kinase p85-alpha; P55G; PI3-kinase p85-gamma subunit; PI3K p85-gamma; Phosphatidylinositol 3-kinase regulatory gamma subunit; PtdIns-3-kinase p85-gamma; p55PIK
Storage and Stability Guarantee
4°C/6 Months
Sample Type
Researchers plate their cell line of choice
Range
At least 5000 cells/well
Assay Time
4.5 hours
References:
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Abstract:
A short protocol of the steps taken in Assay Biotechnology's Cell-Based ELISA Kits.
An illustrated breakdown of the colorimetric Non-Phospho Cell-Based ELISA assay principles.
Western blot analysis of extracts from COS7 cells, treated with H2O2 100uM 30'
Western blot analysis of extracts from COS7 cells treated with H2O2 100uM 30', using PI3-kinase p85-alpha/gamma (Phospho-Tyr467/199) Antibody.
