The Phospho-mTOR(Thr2446) Colorimetric Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can detect Phospho-mTOR(Thr2446) protein expression profile in cells. The kit can be used for measuring the relative amounts of Phospho-mTOR(Thr2446) in cultured cells as well as screening for the effects that variuos treatments, inhibitors (ie siRNA or chemicals), or activators have on Phospho-mTOR(Thr2446).