The Phospho-PKCθ(Ser676) Colorimetric Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can detect Phospho-PKCθ(Ser676) protein expression profile in cells. The kit can be used for measuring the relative amounts of Phospho-PKCθ(Ser676) in cultured cells as well as screening for the effects that variuos treatments, inhibitors (ie siRNA or chemicals), or activators have on Phospho-PKCθ(Ser676).