Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role. The protein encoded by this gene is one of the PKC family members. It is a calcium-independent and phospholipid-dependent protein kinase. This kinase is important for T-cell activation. It is required for the activation of the transcription factors NF-kappaB and AP-1, and may link the T cell receptor (TCR) signaling complex to the activation of the transcription factors. [provided by RefSeq, Jul 2008]
Catalog No
A0804
Reactivity
Human, Rat, Mouse
Applications
WB, IHC-p, IF(paraffin section), ELISA
Modification
Phospho Specific
Source
Polyclonal Rabbit
Dilution
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage and Stability
-20°C/1 year
Other Name
PRKCQ; PRKCT; Protein kinase C theta type; nPKC-theta
Molecular Weight (Da)
81865
Gene Name
PRKCQ
Protein Name
Protein kinase C theta type
Human Gene ID
5588
Human Swiss Prot No.
Q04759
Immunogen
The antiserum was produced against synthesized peptide derived from human PKC thet around the phosphorylation site of Ser695. AA range:657-706
Specificity
Phospho-PKC θ (S695) Polyclonal Antibody detects endogenous levels of PKC θ protein only when phosphorylated at S695.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Western blot analysis of lysates from Jurkat cells treated with EGF 200ng/ml 15', using PKC thet (Phospho-Ser695) Antibody. The lane on the right is blocked with the phospho peptide.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using PKC thet (Phospho-Ser695) Antibody. The picture on the right is blocked with the phospho peptide.