Phospho-B-RAF (Ser446) Colorimetric Cell-Based ELISA Kit
Catalog No :
CBP1739
Reactivity
H:S446,M:S483
Assay Type
Cell-Based ELISA
Format
2 Plates: TC 96-Well Plates
Dynamic Range
At least 5000 cells/well
Size
Price/Quantity
$500
Quantity
Details
Product Name
Phospho-B-RAF (Ser446) Colorimetric Cell-Based ELISA Kit
Description
The Phospho-B-RAF(Ser446) Colorimetric Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can detect Phospho-B-RAF(Ser446) protein expression profile in cells. The kit can be used for measuring the relative amounts of Phospho-B-RAF(Ser446) in cultured cells as well as screening for the effects that variuos treatments, inhibitors (ie siRNA or chemicals), or activators have on Phospho-B-RAF(Ser446).
Catalog No
CBP1739
Format
2 Plates: TC 96-Well Plates
Detection Target
Phospho-B-RAF(Ser446)
Reactivity
H:S446, M:S483
Detection Method
Colorimetric 450 nm
Assay Type
Cell-Based ELISA
SwissProt ID
P15056
NCBI Gene ID
673
Target Synonyms
B-RAF; B-RAF proto-oncogene serine/threonine-protein kinase; B-Raf proto-oncogene serine/threonine-protein kinase; BRAF; BRAF1; c-RMIL; kinase B-Raf; p94; RAFB1; RMIL; RMIL serine/threonine-protein kinase; v-Raf murine sarcoma viral oncogene homolog B1
Storage and Stability Guarantee
4°C/6 Months
Sample Type
Researchers plate their cell line of choice
Range
At least 5000 cells/well
Assay Time
4.5 hours
References:
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Abstract:
A short protocol of the steps taken in Assay Biotechnology's Cell-Based ELISA Kits.
An illustrated breakdown of the Colorimetric Cell-Based ELISA assay principles.
Western blot analysis of extracts from rat brain
Western blot analysis of extracts from 293 cells treated with EGF 200ng/ml 30', using B-RAF (Phospho-Ser446) Antibody.