Reactivity
Human, Mouse
Applications
WB, IHC-p, IF/ICC, ELISA
Modification
Unmodified/Total
Source
Polyclonal Rabbit
Dilution
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage and Stability
-20°C/1 year
Other Name
BRAF; BRAF1; RAFB1; Serine/threonine-protein kinase B-raf; Proto-oncogene B-Raf; p94; v-Raf murine sarcoma viral oncogene homolog B1
Molecular Weight (Da)
84437
Gene Name
BRAF
Protein Name
Serine/threonine-protein kinase B-raf
Human Gene ID
673
Human Swiss Prot No.
P15056
Immunogen
The antiserum was produced against synthesized peptide derived from human B-RAF. AA range:171-220
Specificity
Raf-B Polyclonal Antibody detects endogenous levels of Raf-B protein.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.