phosphoprotein enriched in astrocytes 15(PEA15) Homo sapiens This gene encodes a death effector domain-containing protein that functions as a negative regulator of apoptosis. The encoded protein is an endogenous substrate for protein kinase C. This protein is also overexpressed in type 2 diabetes mellitus, where it may contribute to insulin resistance in glucose uptake. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]
Catalog No
A0545
Reactivity
Human, Mouse, Rat, Monkey
Applications
WB, IHC-p, IF(paraffin section), ELISA
Modification
Phospho Specific
Source
Polyclonal Rabbit
Dilution
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage and Stability
-20°C/1 year
Other Name
PEA15; Astrocytic phosphoprotein PEA-15; 15 kDa phosphoprotein enriched in astrocytes; Phosphoprotein enriched in diabetes; PED
Molecular Weight (Da)
15040
Gene Name
PEA15
Protein Name
Astrocytic phosphoprotein PEA-15
Human Gene ID
8682
Human Swiss Prot No.
Q15121
Immunogen
The antiserum was produced against synthesized peptide derived from human PEA-15 around the phosphorylation site of Ser116. AA range:81-130
Specificity
Phospho-PEA-15 (S116) Polyclonal Antibody detects endogenous levels of PEA-15 protein only when phosphorylated at S116.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Western blot analysis of lysates from COS7 cells treated with INSULIN 0.01U/ML 15', using PEA-15 (Phospho-Ser116) Antibody. The lane on the right is blocked with the phospho peptide.
Immunohistochemistry analysis of paraffin-embedded human skeletal muscle, using PEA-15 (Phospho-Ser116) Antibody. The picture on the right is blocked with the phospho peptide.
