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Primary Antibodies
Raf-1 (Phospho-Ser621) Antibody
Raf-1 (Phospho-Ser621) Antibody

Assay Biotechnology Inc Antibodies and ELISA Kits have been rigorously validated for laboratory grade quality results. High specificity paired with high affinity for targets of interest give researchers an advantage with our products

Catalog No: A0566
Reactivity:Human,Mouse,Rat,Monkey
Applications:WB,IHC-p,IF/ICC,ELISA
Modification:Phospho Specific
Source:Polyclonal Rabbit
Storage and Stability:-20°C/1 year

$205
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Kinases and Phosphatases
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Raf-1 (Phospho-Ser621) Antibody

Western blot analysis of lysates from HeLa cells treated with UV 5', using C-RAF (Phospho-Ser621) Antibody. The lane on the right is blocked with the phospho peptide.

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Product Details
Product Name
Raf-1 (Phospho-Ser621) Antibody
Description
This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2. [provided by RefSeq, Jul 2008]
Catalog No
A0566
Reactivity
Human, Mouse, Rat, Monkey
Applications
WB, IHC-p, IF/ICC, ELISA
Modification
Phospho Specific
Source
Polyclonal Rabbit
Dilution
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage and Stability
-20°C/1 year
Other Name
RAF1; RAF; RAF proto-oncogene serine/threonine-protein kinase; Proto-oncogene c-RAF; cRaf; Raf-1
Molecular Weight (Da)
73052
Gene Name
RAF1
Protein Name
RAF proto-oncogene serine/threonine-protein kinase
Human Gene ID
5894
Human Swiss Prot No.
P04049
Immunogen
The antiserum was produced against synthesized peptide derived from human C-RAF around the phosphorylation site of Ser621. AA range:591-640
Specificity
Phospho-Raf-1 (S621) Polyclonal Antibody detects endogenous levels of Raf-1 protein only when phosphorylated at S621.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
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Primary Antibodies

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    • Research Areas

    Cell Adhesion Proteins Cell Cycle Proteins Channel Proteins Exosome GDP/GTP Binding Proteins Growth Factors and Hormones Homeodomain Proteins Immunohistochemical Kinases and Phosphatases Signaling

     

    Membrane Receptors Neurobiology Signaling Intermediates Steroid Receptors Structural Proteins Synthesis and Degradation Transcription Regulators Transport and Trafficking Tumor Suppressors/Apoptosis
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