This gene encodes one of two sphingosine kinase isozymes that catalyze the phosphorylation of sphingosine into sphingosine 1-phosphate. Sphingosine 1-phosphate mediates many cellular processes including migration, proliferation and apoptosis, and also plays a role in several types of cancer by promoting angiogenesis and tumorigenesis. The encoded protein may play a role in breast cancer proliferation and chemoresistance. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2011]
Catalog No
A8423
Reactivity
Human, Mouse, Rat
Applications
IHC-p, IF(paraffin section), WB, ELISA
Modification
Phospho Specific
Source
Polyclonal Rabbit
Dilution
WB 1:500-2000 Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage and Stability
-20°C/1 year
Other Name
SPHK2; Sphingosine kinase 2; SK 2; SPK 2
Molecular Weight (Da)
69217
Gene Name
SPHK2
Protein Name
Sphingosine kinase 2
Human Gene ID
56848
Human Swiss Prot No.
Q9NRA0
Immunogen
The antiserum was produced against synthesized peptide derived from human SPHK2 around the phosphorylation site of Thr614. AA range:580-629
Specificity
Phospho-SphK2 (T614) Polyclonal Antibody detects endogenous levels of SphK2 protein only when phosphorylated at T614.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using SPHK2 (Phospho-Thr614) Antibody. The picture on the right is blocked with the phospho peptide.
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using SPHK2 (Phospho-Thr614) Antibody
