This gene encodes a transcription factor that binds to the sterol regulatory element-1 (SRE1), which is a decamer flanking the low density lipoprotein receptor gene and some genes involved in sterol biosynthesis. The protein is synthesized as a precursor that is attached to the nuclear membrane and endoplasmic reticulum. Following cleavage, the mature protein translocates to the nucleus and activates transcription by binding to the SRE1. Sterols inhibit the cleavage of the precursor, and the mature nuclear form is rapidly catabolized, thereby reducing transcription. The protein is a member of the basic helix-loop-helix-leucine zipper (bHLH-Zip) transcription factor family. This gene is located within the Smith-Magenis syndrome region on chromosome 17. [provided by RefSeq, Mar 2016]
Catalog No
A8425
Reactivity
Human, Rat
Applications
WB, IHC-p, IF(paraffin section), ELISA
Modification
Phospho Specific
Source
Polyclonal Rabbit
Dilution
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage and Stability
-20°C/1 year
Other Name
SREBF1; BHLHD1; SREBP1; Sterol regulatory element-binding protein 1; SREBP-1; Class D basic helix-loop-helix protein 1; bHLHd1; Sterol regulatory element-binding transcription factor 1
Molecular Weight (Da)
121675
Gene Name
SREBF1
Protein Name
Sterol regulatory element-binding protein 1
Human Gene ID
6720
Human Swiss Prot No.
P36956
Immunogen
The antiserum was produced against synthesized peptide derived from human SREBP-1 around the phosphorylation site of Ser439. AA range:405-454
Specificity
Phospho-SREBP-1 (S439) Polyclonal Antibody detects endogenous levels of SREBP-1 protein only when phosphorylated at S439.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Western blot analysis of lysates from Jurkat cells treated with TNF 20ng/ml 30', using SREBP-1 (Phospho-Ser439) Antibody. The lane on the right is blocked with the phospho peptide.
Immunohistochemistry analysis of paraffin-embedded human brain, using SREBP-1 (Phospho-Ser439) Antibody. The picture on the right is blocked with the phospho peptide.
