Reactivity
Human, Mouse, Rat
Applications
WB, IHC-p, IF/ICC, ELISA
Modification
Unmodified/Total
Source
Polyclonal Rabbit
Dilution
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage and Stability
-20°C/1 year
Other Name
SIAH1; HUMSIAH; E3 ubiquitin-protein ligase SIAH1; Seven in absentia homolog 1; Siah-1; Siah-1a; SIAH2; E3 ubiquitin-protein ligase SIAH2; Seven in absentia homolog 2; Siah-2; hSiah2
Molecular Weight (Da)
31123
Gene Name
SIAH1/SIAH2
Protein Name
E3 ubiquitin-protein ligase SIAH1/2
Human Gene ID
6477/6478
Human Swiss Prot No.
Q8IUQ4/O43255
Immunogen
The antiserum was produced against synthesized peptide derived from human SIAH1. AA range:181-230
Specificity
Siah-1/2 Polyclonal Antibody detects endogenous levels of Siah-1/2 protein.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.