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Primary Antibodies
Phospho-Raf1-S259 Rabbit mAb
Phospho-Raf1-S259 Rabbit mAb

Assay Biotechnology Inc Antibodies and ELISA Kits have been rigorously validated for laboratory grade quality results. High specificity paired with high affinity for targets of interest give researchers an advantage with our products

Catalog No: M1225
Reactivity:Human,Rat
Applications:WB,ELISA
Modifcation:Phospho S259
Source:Rabbit
Storage and Stability:Store at -20℃/ 1 year. Avoid freeze / thaw cycles.

$150
# SDS #
Recombinant Antibody
20ul Size Available
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Phospho-Raf1-S259 Rabbit mAb

Western blot analysis of various lysates using Phospho-Raf1-S259 Rabbit mAb (AP1012) at 1:1000 dilution. Both 293T cells and C6 cells were treated by Forskolin (10 uM) at 37℃ for 1 hour after serum-starvation overnight.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.

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Product Details
Product Name
Phospho-Raf1-S259 Rabbit mAb
Description
This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2.
Catalog No
M1225
Reactivity
Human, Rat
Applications
WB, ELISA
Application Dilutions
WB,1:500 - 1:1000 | ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Modifcation
Phospho S259
Source
Rabbit
Isotype
IgG
Immunogen
A synthetic phosphorylated peptide around S259 of human RAF1 (P04049).
Calculated MW (kDa)
73kDa
Observed MW (kDa)Gene Name
73kDa/75kDaRAF1
Other Name
NS5; CRAF; Raf-1; c-Raf; CMD1NN; Phospho-Raf1-S259
Gene ID
5894
Human Swiss Prot
P04049
Formulation
PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Storage and Stability
Store at -20℃/ 1 year. Avoid freeze / thaw cycles.
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Primary Antibodies

    • ELISA Kits

    • Research Areas

    Cell Adhesion Proteins Cell Cycle Proteins Channel Proteins Exosome GDP/GTP Binding Proteins Growth Factors and Hormones Homeodomain Proteins Immunohistochemical Kinases and Phosphatases Signaling

     

    Membrane Receptors Neurobiology Signaling Intermediates Steroid Receptors Structural Proteins Synthesis and Degradation Transcription Regulators Transport and Trafficking Tumor Suppressors/Apoptosis
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