The Tfact E2F-5 TFact™ DNA-Binding ELISA contains components necessary for detection and qualitative analysis of E2F-5 active transcription factors in eukaryotic nuclear or cell lysates. Tfact ELISA kits are designed to significantly reduce experiment time and ensure sensitivity and flexibility for high-throughput screening.
The TFact™ E2F-5 DNA-Binding ELISA detects active E2F-5 in Jurkat Nuclear Extract. The Jurkat cells were grown 3 days in RPMI 1640 with 10% FBS and harvested for nuclear extract.