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> Products > Primary Antibodies > MDM2 Antibody

MDM2 Antibody

Overview Description
Datasheet SDS
   Tumor Suppressors/Apoptosis   
Catalog No : C30111
  • Reactivity
    Human,Rat,Mouse
  • Applications
    IF/ICC,WB,IHC-p,ELISA
  • Modification
    Unmodified/Total
  • Source
    Polyclonal Rabbit
  • Storage and Stability
    -20°C/1 year
  • Size
    50 μl
  • Price/Quantity
    $205
  • Quantity
Add to Cart
Details
  • Product Name
    MDM2 Antibody
  • Description
    This gene encodes a nuclear-localized E3 ubiquitin ligase. The encoded protein can promote tumor formation by targeting tumor suppressor proteins, such as p53, for proteasomal degradation. This gene is itself transcriptionally-regulated by p53. Overexpression or amplification of this locus is detected in a variety of different cancers. There is a pseudogene for this gene on chromosome 2. Alternative splicing results in a multitude of transcript variants, many of which may be expressed only in tumor cells. [provided by RefSeq, Jun 2013]
  • Catalog No
    C30111
  • Reactivity
    Human, Rat, Mouse
  • Applications
    IF/ICC, WB, IHC-p, ELISA
  • Modification
    Unmodified/Total
  • Source
    Polyclonal Rabbit
  • Dilution
    IF: 1:50-200 Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.
  • Purification
    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • Concentration
    1 mg/ml
  • Storage and Stability
    -20°C/1 year
  • Other Name
    MDM2; E3 ubiquitin-protein ligase Mdm2; Double minute 2 protein; Hdm2; Oncoprotein Mdm2; p53-binding protein Mdm2
  • Molecular Weight (Da)
    55233
  • Gene Name
    MDM2
  • Protein Name
    E3 ubiquitin-protein ligase Mdm2
  • Human Gene ID
    4193
  • Human Swiss Prot No.
    Q00987
  • Immunogen
    The antiserum was produced against synthesized peptide derived from the C-terminal region of human MDM2. AA range:381-430
  • Specificity
    MDM2 Polyclonal Antibody detects endogenous levels of MDM2 protein.
  • Formulation
    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
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Application Data
Western blot analysis of lysate from mouse liver cells, using MDM2 Antibody.
Application Data
Immunohistochemical analysis of paraffin-embedded human-kidney, antibody was diluted at 1:100
Application Data
Immunohistochemical analysis of paraffin-embedded human-colon, antibody was diluted at 1:100
Application Data
Immunohistochemical analysis of paraffin-embedded human-colon, antibody was diluted at 1:100
Application Data
Western Blot analysis of mouse liver cells using MDM2 Polyclonal Antibody. Antibody was diluted at 1:2000. Secondary antibody(catalogNo.:SA0002) was diluted at 1:20000
Application Data
Immunohistochemical analysis of paraffin-embedded Human-stomach-cancer tissue. 1,MDM2 Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Application Data
Immunohistochemical analysis of paraffin-embedded Human-kidney tissue. 1,MDM2 Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Application Data
Immunohistochemical analysis of paraffin-embedded Human-lung-cancer tissue. 1,MDM2 Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Application Data
Immunohistochemical analysis of paraffin-embedded Human-lung tissue. 1,MDM2 Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Application Data
Immunohistochemical analysis of paraffin-embedded Human-liver-cancer tissue. 1,MDM2 Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Application Data
Immunohistochemical analysis of paraffin-embedded Human-breast-cancer tissue. 1,MDM2 Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Application Data
Immunofluorescence analysis of human-kidney-cancer tissue. 1,MDM2 Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Application Data
Immunofluorescence analysis of human-liver-cancer tissue. 1,MDM2 Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Primary Antibodies

    • ELISA Kits

    • Research Areas

    Cell Adhesion Proteins Cell Cycle Proteins Channel Proteins Exosome GDP/GTP Binding Proteins Growth Factors and Hormones Homeodomain Proteins Immunohistochemical Kinases and Phosphatases Signaling

     

    Membrane Receptors Neurobiology Signaling Intermediates Steroid Receptors Structural Proteins Synthesis and Degradation Transcription Regulators Transport and Trafficking Tumor Suppressors/Apoptosis
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