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Primary Antibodies
ACTN1 Rabbit mAb
ACTN1 Rabbit mAb

Assay Biotechnology Inc Antibodies and ELISA Kits have been rigorously validated for laboratory grade quality results. High specificity paired with high affinity for targets of interest give researchers an advantage with our products

Catalog No: M0284
Reactivity:Human,Mouse
Applications:WB,ELISA
Modifcation:Unmodified
Source:Rabbit
Storage and Stability:Store at -20℃/ 1 year. Avoid freeze / thaw cycles.

$150
# Datasheet #
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Recombinant Antibody
20ul Size Available
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ACTN1 Rabbit mAb

Western blot analysis of various lysates using ACTN1 Rabbit mAb (A26291) at 1:3000 dilution incubated overnight at 4℃.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Negative control (NC): Daudi|Exposure time: 60s.

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Product Details
Product Name
ACTN1 Rabbit mAb
Description
Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, cytoskeletal, alpha actinin isoform and maps to the same site as the structurally similar erythroid beta spectrin gene. Three transcript variants encoding different isoforms have been found for this gene.
Catalog No
M0284
Reactivity
Human, Mouse
Applications
WB, ELISA
Application Dilutions
WB,1:1500 - 1:6000 | ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Modifcation
Unmodified
Source
Rabbit
Isotype
IgG
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 281-380 of human α-Actinin (NP_001093.1).
Calculated MW (kDa)
103kDa
Observed MW (kDa)Gene Name
103kDaACTN1
Other Name
BDPLT15
Gene ID
87
Human Swiss Prot
P12814
Formulation
PBS with 0.09% Sodium azide,0.05% BSA,50% glycerol,pH7.3.
Storage and Stability
Store at -20℃/ 1 year. Avoid freeze / thaw cycles.
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Primary Antibodies

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