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Primary Antibodies
T-bet / Tbx21 Rabbit mAb
T-bet / Tbx21 Rabbit mAb

Assay Biotechnology Inc Antibodies and ELISA Kits have been rigorously validated for laboratory grade quality results. High specificity paired with high affinity for targets of interest give researchers an advantage with our products

Catalog No: M0270
Reactivity:Human,Mouse,Rat
Applications:WB,IP,ELISA
Modifcation:Unmodified
Source:Rabbit
Storage and Stability:Store at -20℃/ 1 year. Avoid freeze / thaw cycles.

$150
# Datasheet #
# SDS #
Recombinant Antibody
20ul Size Available
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T-bet / Tbx21 Rabbit mAb

Western blot analysis of various lysates using T-bet / Tbx21 Rabbit mAb (A27203) at 1:1000 dilution incubated overnight at 4℃.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Negative control (NC): U-937|Exposure time: 180s.

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Product Details
Product Name
T-bet / Tbx21 Rabbit mAb
Description
This gene is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. This gene is the human ortholog of mouse Tbx21/Tbet gene. Studies in mouse show that Tbx21 protein is a Th1 cell-specific transcription factor that controls the expression of the hallmark Th1 cytokine, interferon-gamma (IFNG). Expression of the human ortholog also correlates with IFNG expression in Th1 and natural killer cells, suggesting a role for this gene in initiating Th1 lineage development from naive Th precursor cells.
Catalog No
M0270
Reactivity
Human, Mouse, Rat
Applications
WB, IP, ELISA
Application Dilutions
WB,1:500 - 1:1000 | IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells | ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
Modifcation
Unmodified
Source
Rabbit
Isotype
IgG
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 322-535 of human T-bet/Tbx21(NP_037483.1).
Calculated MW (kDa)
58kDa
Observed MW (kDa)Gene Name
65kDa/58-68KDTBX21
Other Name
TBET; IMD88; T-PET; T-bet; TBLYM
Gene ID
30009
Human Swiss Prot
Q9UL17
Formulation
PBS with 0.09% Sodium azide,0.05% BSA,50% glycerol,pH7.3.
Storage and Stability
Store at -20℃/ 1 year. Avoid freeze / thaw cycles.
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Primary Antibodies

    • ELISA Kits

    • Research Areas

    Cell Adhesion Proteins Cell Cycle Proteins Channel Proteins Exosome GDP/GTP Binding Proteins Growth Factors and Hormones Homeodomain Proteins Immunohistochemical Kinases and Phosphatases Signaling

     

    Membrane Receptors Neurobiology Signaling Intermediates Steroid Receptors Structural Proteins Synthesis and Degradation Transcription Regulators Transport and Trafficking Tumor Suppressors/Apoptosis
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