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Primary Antibodies
SLC12A1 / NKCC2 Rabbit mAb
SLC12A1 / NKCC2 Rabbit mAb

Assay Biotechnology Inc Antibodies and ELISA Kits have been rigorously validated for laboratory grade quality results. High specificity paired with high affinity for targets of interest give researchers an advantage with our products

Catalog No: M0165
Reactivity:Human,Mouse,Rat
Applications:WB,ELISA
Modifcation:Unmodified
Source:Rabbit
Storage and Stability:Store at -20℃/ 1 year. Avoid freeze / thaw cycles.

$150
# Datasheet #
# SDS #
Recombinant Antibody
20ul Size Available
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SLC12A1 / NKCC2 Rabbit mAb

Western blot analysis of various lysates using SLC12A1 / NKCC2 Rabbit mAb (A20933) at 1:1000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 60s.

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Product Details
Product Name
SLC12A1 / NKCC2 Rabbit mAb
Description
This gene encodes a kidney-specific sodium-potassium-chloride cotransporter that is expressed on the luminal membrane of renal epithelial cells of the thick ascending limb of Henle's loop and the macula densa. It plays a key role in concentrating urine and accounts for most of the NaCl resorption. It is sensitive to such diuretics as furosemide and bumetanide. Some Bartter-like syndromes result from defects in this gene. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional splice variants have been described but their biological validity in humans has not been experimentally proven.
Catalog No
M0165
Reactivity
Human, Mouse, Rat
Applications
WB, ELISA
Application Dilutions
WB,1:500 - 1:1000 | ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Modifcation
Unmodified
Source
Rabbit
Isotype
IgG
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 601-800 of human SLC12A1 / NKCC2 (Q13621).
Calculated MW (kDa)
121kDa
Observed MW (kDa)Gene Name
100-290kDaSLC12A1
Other Name
BSC; BSC1; CCC2; BSC-1; NKCC2; SLC12A1 / NKCC2
Gene ID
6557
Human Swiss Prot
Q13621
Formulation
PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Storage and Stability
Store at -20℃/ 1 year. Avoid freeze / thaw cycles.
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