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Phospho-eIF4EBP1-S65 Rabbit mAb
Phospho-eIF4EBP1-S65 Rabbit mAb

Assay Biotechnology Inc Antibodies and ELISA Kits have been rigorously validated for laboratory grade quality results. High specificity paired with high affinity for targets of interest give researchers an advantage with our products

Catalog No: M0147
Reactivity:Human,Mouse
Applications:WB,ELISA
Modifcation:Phospho S65
Source:Rabbit
Storage and Stability:Store at -20℃/ 1 year. Avoid freeze / thaw cycles.

$150
# Datasheet #
# SDS #
Recombinant Antibody
20ul Size Available
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Phospho-eIF4EBP1-S65 Rabbit mAb

Western blot analysis of various lysates using Phospho-eIF4EBP1-S65 Rabbit mAb (AP1313) at1:20000 dilution. 293T cells were treated by IGF-1 (50 ng/ml) at 37℃ for 5 minutes after serum-starvation overnight.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.

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Product Details
Product Name
Phospho-eIF4EBP1-S65 Rabbit mAb
Description
This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.
Catalog No
M0147
Reactivity
Human, Mouse
Applications
WB, ELISA
Application Dilutions
WB,1:2000 - 1:20000 | ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Modifcation
Phospho S65
Source
Rabbit
Isotype
IgG
Immunogen
A synthetic phosphorylated peptide around S65 of human Phospho-EIF4EBP1-S65 (NP_004086.1).
Calculated MW (kDa)
13kDa
Observed MW (kDa)Gene Name
15-20kDaEIF4EBP1
Other Name
BP-1; 4EBP1; 4E-BP1; PHAS-I; Phospho-eIF4EBP1-S65
Gene ID
1978
Human Swiss Prot
Q13541
Formulation
PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Storage and Stability
Store at -20℃/ 1 year. Avoid freeze / thaw cycles.
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