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Phospho-EGFR-Y845 Rabbit mAb
Phospho-EGFR-Y845 Rabbit mAb

Assay Biotechnology Inc Antibodies and ELISA Kits have been rigorously validated for laboratory grade quality results. High specificity paired with high affinity for targets of interest give researchers an advantage with our products

Catalog No: M0104
Reactivity:Human
Applications:WB,ELISA
Modifcation:Phospho Y845
Source:Rabbit
Storage and Stability:Store at -20℃/ 1 year. Avoid freeze / thaw cycles.

$150
# Datasheet #
# SDS #
Recombinant Antibody
20ul Size Available
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Phospho-EGFR-Y845 Rabbit mAb

Western blot analysis of lysates from A-431 cells, using Phospho-EGFR-Y845 (AP1425) at 1:800 dilution. A-431 cells were treated by EGF (200 ng/ml) at 37℃ for 30 minutes.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 180s.

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Product Details
Product Name
Phospho-EGFR-Y845 Rabbit mAb
Description
The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor, thus inducing receptor dimerization and tyrosine autophosphorylation leading to cell proliferation. Mutations in this gene are associated with lung cancer. EGFR is a component of the cytokine storm which contributes to a severe form of Coronavirus Disease 2019 (COVID-19) resulting from infection with severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2).
Catalog No
M0104
Reactivity
Human
Applications
WB, ELISA
Application Dilutions
WB,1:500 - 1:1000 | ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Modifcation
Phospho Y845
Source
Rabbit
Isotype
IgG
Immunogen
A phospho synthetic peptide corresponding to residues surrounding Y845 of Human EGFR.
Calculated MW (kDa)
134kDa
Observed MW (kDa)Gene Name
175kDaEGFR
Other Name
ERBB; ERRP; HER1; mENA; ERBB1; PIG61; NISBD2; Phospho-EGFR (Y845)
Gene ID
1956
Human Swiss Prot
P00533
Formulation
PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Storage and Stability
Store at -20℃/ 1 year. Avoid freeze / thaw cycles.
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Primary Antibodies

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    • Research Areas

    Cell Adhesion Proteins Cell Cycle Proteins Channel Proteins Exosome GDP/GTP Binding Proteins Growth Factors and Hormones Homeodomain Proteins Immunohistochemical Kinases and Phosphatases Signaling

     

    Membrane Receptors Neurobiology Signaling Intermediates Steroid Receptors Structural Proteins Synthesis and Degradation Transcription Regulators Transport and Trafficking Tumor Suppressors/Apoptosis
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