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Phospho-VEGF Receptor 2-Y1175 Rabbit mAb
Phospho-VEGF Receptor 2-Y1175 Rabbit mAb

Assay Biotechnology Inc Antibodies and ELISA Kits have been rigorously validated for laboratory grade quality results. High specificity paired with high affinity for targets of interest give researchers an advantage with our products

Catalog No: M0086
Reactivity:Human
Applications:WB,ELISA
Modifcation:Phospho Y1175
Source:Rabbit
Storage and Stability:Store at -20℃/ 1 year. Avoid freeze / thaw cycles.

$150
# Datasheet #
# SDS #
Recombinant Antibody
20ul Size Available
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Phospho-VEGF Receptor 2-Y1175 Rabbit mAb

Western blot analysis of lysates from A-431 lysates cells, using Phospho-VEGF Receptor 2-Y1175 Rabbit mAb (AP1385) at 1:3000 dilution. A-431 cells were treated by EGF (100 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.

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Product Details
Product Name
Phospho-VEGF Receptor 2-Y1175 Rabbit mAb
Description
Vascular endothelial growth factor (VEGF) is a major growth factor for endothelial cells. This gene encodes one of the two receptors of the VEGF. This receptor, known as kinase insert domain receptor, is a type III receptor tyrosine kinase. It functions as the main mediator of VEGF-induced endothelial proliferation, survival, migration, tubular morphogenesis and sprouting. The signalling and trafficking of this receptor are regulated by multiple factors, including Rab GTPase, P2Y purine nucleotide receptor, integrin alphaVbeta3, T-cell protein tyrosine phosphatase, etc.. Mutations of this gene are implicated in infantile capillary hemangiomas.
Catalog No
M0086
Reactivity
Human
Applications
WB, ELISA
Application Dilutions
WB,1:500 - 1:1000 | ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Modifcation
Phospho Y1175
Source
Rabbit
Isotype
IgG
Immunogen
A synthetic phosphorylated peptide around Y1175 of human VEGF Receptor 2.
Calculated MW (kDa)
152kDa
Observed MW (kDa)Gene Name
230kDaKDR
Other Name
FLK1; CD309; VEGFR; VEGFR2; Phospho-VEGF Receptor 2-Y1175
Gene ID
3791
Human Swiss Prot
P35968
Formulation
PBS with 0.09% Sodium azide,0.05% BSA,50% glycerol,pH7.3.
Storage and Stability
Store at -20℃/ 1 year. Avoid freeze / thaw cycles.
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Primary Antibodies

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