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Phospho-TBK1/NAK-S172 Rabbit mAb
Phospho-TBK1/NAK-S172 Rabbit mAb

Assay Biotechnology Inc Antibodies and ELISA Kits have been rigorously validated for laboratory grade quality results. High specificity paired with high affinity for targets of interest give researchers an advantage with our products

Catalog No: M0007
Reactivity:Human,Mouse
Applications:WB,ELISA
Modifcation:Phospho S172
Source:Rabbit
Storage and Stability:Store at -20℃/ 1 year. Avoid freeze / thaw cycles.

$150
# Datasheet #
# SDS #
Recombinant Antibody
20ul Size Available
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Phospho-TBK1/NAK-S172 Rabbit mAb

Western blot analysis of lysates from HeLa cells using Phospho-TBK1/NAK-S172 Rabbit mAb (AP1418) at 1:1000 dilution incubated overnight at 4℃. HeLa cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 30 μg per lane.| Blocking buffer: 3 % nonfat dry milk in TBST.| Detection: ECL Basic Kit (RM00020).| Exposure time: 90s.

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Product Details
Product Name
Phospho-TBK1/NAK-S172 Rabbit mAb
Description
The NF-kappa-B (NFKB) complex of proteins is inhibited by I-kappa-B (IKB) proteins, which inactivate NFKB by trapping it in the cytoplasm. Phosphorylation of serine residues on the IKB proteins by IKB kinases marks them for destruction via the ubiquitination pathway, thereby allowing activation and nuclear translocation of the NFKB complex. The protein encoded by this gene is similar to IKB kinases and can mediate NFKB activation in response to certain growth factors. The protein is also an important kinase for antiviral innate immunity response.
Catalog No
M0007
Reactivity
Human, Mouse
Applications
WB, ELISA
Application Dilutions
WB,1:1000 - 1:2000 | ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Modifcation
Phospho S172
Source
Rabbit
Isotype
IgG
Immunogen
A phospho synthetic peptide corresponding to residues surrounding S172 of Human TBK1/NAK.
Calculated MW (kDa)
84kDa
Observed MW (kDa)Gene Name
84kDaTBK1
Other Name
NAK; T2K; IIAE8; FTDALS4; Phospho-TBK1/NAK-S172
Gene ID
29110
Human Swiss Prot
Q9UHD2
Formulation
PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Storage and Stability
Store at -20℃/ 1 year. Avoid freeze / thaw cycles.
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Primary Antibodies

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    Cell Adhesion Proteins Cell Cycle Proteins Channel Proteins Exosome GDP/GTP Binding Proteins Growth Factors and Hormones Homeodomain Proteins Immunohistochemical Kinases and Phosphatases Signaling

     

    Membrane Receptors Neurobiology Signaling Intermediates Steroid Receptors Structural Proteins Synthesis and Degradation Transcription Regulators Transport and Trafficking Tumor Suppressors/Apoptosis
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