TEV Protease (with His-tag)

Assay Biotechnology Inc Antibodies and ELISA Kits have been rigorously validated for laboratory grade quality results. High specificity paired with high affinity for targets of interest give researchers an advantage with our products

Catalog No: YS0242

Concentration:10 U/μl

Size:

Price/Quantity $95

Quantity:

Datasheet

TEV Protease (with His-tag)

Molecular Biology Products by Assay Biotechnology Inc.

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Product Details

Product Name

TEV Protease (with His-tag)

Description

TEV Protease (with His-tag) is derived from the tobacco etch virus and has been genetically engineered for recombinant expression in Escherichia coli. It retains the natural enzymatic activity of TEV protease while exhibiting improved tolerance to variations in pH and temperature. TEV Protease is a highly specific cysteine protease, widely utilized for the removal of affinity tags from fusion proteins. It precisely recognizes the seven-amino-acid sequence EXXYXQ↓(G/S) and cleaves between the glutamine and glycine/serine residues. The most commonly recognized sequence is Glu-Asn-Leu-Tyr-Phe-Gln↓Gly. The inclusion of a His-tag at the N-terminus of TEV Protease allows for its efficient removal via Ni-affinity chromatography following digestion, thereby simplifying the purification of the target protein.

Catalog No

YS0242

Application

Cleave off the affinity tag from the fusion protein.

Properties

Specifically recognizes and cleaves the heptapeptide sequence EXXYXQ↓(G/S).