PNGase F (with His-tag)

Assay Biotechnology Inc Antibodies and ELISA Kits have been rigorously validated for laboratory grade quality results. High specificity paired with high affinity for targets of interest give researchers an advantage with our products

Catalog No: YS0240

Concentration:500 U/μl

Size:

Price/Quantity $245

Quantity:

Datasheet

PNGase F (with His-tag)

Molecular Biology Products by Assay Biotechnology Inc.

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Antibodies 1-2 business day, ELISA kits 2-3 business day lead time

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Product Details

Product Name

PNGase F (with His-tag)

Description

PNGase F (Peptide N-Glycosidase F) is a high effective amidase derived from Elizabethkingia miricola. It cleaves asparagine-linked high mannose as well as hybrid and complex oligosaccharides from glycoproteins. The cleavage site of PNGase F is the amide bond between the N-acetylglucosamine (GlcNAc) inside the protein and the asparagine residue, while converting the asparagine residue in the digested protein to aspartic acid. This product is expressed through recombinant expression in E.coli, and it contains a His tag and 50% glycerol. It is commonly used for deglycosylation of antibodies and related proteins.

Catalog No

YS0240

Application

Commonly used for deglycosylation of antibodies and related proteins.

Properties

Efficiently cleaves N-linked glycan chains of animal cell glycoproteins, with a His tag and no glycerol, does not affect subsequent analysis.