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SpCas9-NLS

Assay Biotechnology Inc Antibodies and ELISA Kits have been rigorously validated for laboratory grade quality results. High specificity paired with high affinity for targets of interest give researchers an advantage with our products

Catalog No: YS0222
Concentration:10 μM

$39
Product Details
Product Name
SpCas9-NLS
Description
SpCas9-NLS is an RNA-guided endonuclease originating from Streptococcus pyogenes. This CRISPR-Cas9 system directs the enzyme to specific DNA targets through a guide RNA complex, which comprises crRNA and tracrRNA (or their chimeric single-guide RNA/sgRNA configuration). The nuclease recognizes genomic loci by complementary base pairing between the sgRNA spacer sequence and the target DNA, with PAM (protospacer adjacent motif) dependency located 3 nucleotides upstream of the cleavage site. Notably, the NGG triplet (5'-NGG-3') serves as the canonical PAM recognized by this system. For enhanced intracellular delivery and transcriptional efficiency, the recombinant protein is engineered with an N-terminal nuclear localization signal (NLS) derived from the simian virus 40 (SV40) T antigen. Beyond its primary application in genome editing, SpCas9-NLS is widely employed in vitro for targeted DNA cleavage and molecular cloning protocols.
Catalog No
YS0222
Application
Gene editing, in vitro target DNA cleavage and gene cloning, among others.
Properties
RNA-mediated nuclease with an N-terminal simian virus 40 (SV40) T antigen nuclear localization sequence (NLS).
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