T5 Exonuclease

Assay Biotechnology Inc Antibodies and ELISA Kits have been rigorously validated for laboratory grade quality results. High specificity paired with high affinity for targets of interest give researchers an advantage with our products

Catalog No: YS0214

Concentration:10 U/μl

Size:

Price/Quantity $116

Quantity:

Datasheet

T5 Exonuclease

Molecular Biology Products by Assay Biotechnology Inc.

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Product Details

Product Name

T5 Exonuclease

Description

T5 Exonuclease is a nucleic acid exonuclease that digests DNA from the 5' end in the 5'→3' direction for both double-stranded and single-stranded DNA. It is capable of digesting DNA at nicks or gaps in linear or circular double-stranded DNA,however, it cannot digest supercoiled DNA. In addition, when the Mg²⁺ concentration in the solution is below 1 mM, the activity of T5 Exonuclease towards single-stranded DNA is inhibited. Consequently, T5 Exonuclease is particularly suitable for degrading linearized and nicked plasmids, removing non-circular DNA from ligation products, seamless cloning (Gibson Assembly), and other applications.

Catalog No

YS0214

Application

Degrading linearized and nicked plasmids, removing non-circular DNA from ligation products, seamless cloning (e.g., Gibson Assembly), etc.

Properties

Double-stranded DNA exonuclease and single-stranded DNA endonuclease do not degrade supercoiled double-stranded DNA.