HiFi Taq DNA Ligase

Assay Biotechnology Inc Antibodies and ELISA Kits have been rigorously validated for laboratory grade quality results. High specificity paired with high affinity for targets of interest give researchers an advantage with our products

Catalog No: YS0212

Concentration:N/A

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Price/Quantity $133

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Datasheet

HiFi Taq DNA Ligase

Molecular Biology Products by Assay Biotechnology Inc.

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Lead Time

Antibodies 1-2 business day, ELISA kits 2-3 business day lead time

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408-747-0185

Fax

408-747-0145

order@assaybiotech.com

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Product Details

Product Name

HiFi Taq DNA Ligase

Description

HiFi Taq DNA Ligase is a mutant type of Taq DNA ligase, which has higher ligation efficiency and high fidelity compared with the wild type. Meanwhile, it maintains the basic functions of the wild type and is a high-temperature-resistant ligase with activity in the range of 37~75℃ .Taq DNA ligase, with NAD+ as the coenzyme factor, catalyzes the formation of a phosphodiester bond between the 5'-phosphate and 3'-hydroxyl groups of two adjacent oligonucleotide chains hybridized to the same complementary target DNA strand, and it has significant activity only for DNA nicks. This product is applicable to molecular diagnostic reactions that rely on nick junction, such as ligase detection reaction (LDR), multiplex ligation-dependent probe amplification (MLPA), etc.

Catalog No

YS0212

Application

Suitable for molecular diagnostic applications such as LCR, LDR, and MLPA, but not suitable for molecular cloning or library preparation.

Properties

High fidelity, High temperature -resistant, and has good activity within the range of 37~75°C.