Alkaline Phosphatase (Fast)

Assay Biotechnology Inc Antibodies and ELISA Kits have been rigorously validated for laboratory grade quality results. High specificity paired with high affinity for targets of interest give researchers an advantage with our products

Catalog No: YS0204

Concentration:1 U/μl

Size:

Price/Quantity $105

Quantity:

Datasheet

Alkaline Phosphatase (Fast)

Molecular Biology Products by Assay Biotechnology Inc.

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Product Details

Product Name

Alkaline Phosphatase (Fast)

Description

Alkaline Phosphatase (Fast) is an enzyme that catalyzes the hydrolysis of the 5' and 3' phosphate groups of DNA, RNA, and nucleotides. It can also remove protein phosphate groups but does not hydrolyze phosphodiester and phosphotriester bonds. This enzyme can dephosphorylate the ends of all types of DNA in 10 minutes at 37℃ . It has 100% activity in CutOne® buffer and is inactivated by incubation at 80 ℃ for 20 minutes. When used together with LightiNing® restriction endonucleases, it simplifies the "digestion-dephosphorylation" reaction in one tube.

Catalog No

YS0204

Application

Dephosphorylation, RNA Cloning

Properties

It has 100% activity in the CutOne buffer and can be heat-inactivated. When performing an "enzymatic digestion - dephosphorylation" reaction with the LightiNing endonuclease, it can be completed in the same tube.