The 2× S705 HiFi Master Mix is a ready-to-use 2× premix that contains S705 High-Fidelity DNA Polymerase, dNTPs, and a optimized reaction buffer for PCR. It only requires the addition of template, primers, and Nuclease-Free Water to perform high-fidelity PCR amplification. S705 High-Fidelity DNA Polymerase is a high-fidelity DNA polymerase obtained through directed evolution. Its fidelity is 70-fold higher than that of Taq polymerase, and it is amplification speed is five times faster than that of Pfu polymerase. The 2× S705 HiFi Master Mix is formulated with unique extension factors and specificity enhancers, which significantly improves the capacity for long fragment amplification, specificity, and product yield. When using simple templates such as λDNA and plasmids, the 2× S705 HiFi Master Mix can easily amplify fragments up to 20 kb in length. When using complex templates like genomic DNA, it can effectively amplify fragments up to 12 kb. Furthermore, the 2× S705 HiFi Master Mix demonstrates good tolerance to PCR inhibitors, enabling excellent amplification even from crude templates.