E-Cadherein, 120kDa, is a critical regulator of epithelial junction formation. The E-cadherin internal domain binds with alpha, beta, gamma, and p120 catenins to anchor the ECAD complex to the actin cytoskeleton of the cell. E-cadherin is expressed in virtually all epithelial cells with the exception of adrenocortical cells. The expression in liver cells is weaker than in most other epithelia. ECAD is also expressed in melanocytes (adhering to squamous epithelial cells). Mesothelial cells and granulosa cells are ECAD negative. Mesenchymal cells and neural crest cells are mostly negative but some types may show a weak reaction. Testicular germinative cells are weakly positive. At present, it is commonly used to differentiate breast lobular cancer from ductal cancer (the former is often negative, the latter is often positive).