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Primary Antibodies
α smooth muscle actin (PT0074R) PT® Rabbit mAb
α smooth muscle actin (PT0074R) PT® Rabbit mAb

Assay Biotechnology Inc Antibodies and ELISA Kits have been rigorously validated for laboratory grade quality results. High specificity paired with high affinity for targets of interest give researchers an advantage with our products

Catalog No: YM8040
Reactivity:Human,Mouse,Rat
Applications:WB,IHC,IF,IP,ELISA
Modification:Not Modified
Source:Rabbit IgG,Kappa
Storage and Stability:Store at -20°C/1 year

$190
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Recombinant Antibody
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α smooth muscle actin (PT0074R) PT® Rabbit mAb

Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-α smooth muscle Actin (PT0074R) antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: Hela Lane 2: NIH-3T3 Lane 3: Rat lung Lane 4: C6 Predicted band size: 42kDa Observed band size: 42kDa

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Product Details
Product Name
α smooth muscle actin (PT0074R) PT® Rabbit mAb
Description
function:Seems to play an essential role in cold-induced suppression of cell proliferation.,induction:By cold stress an in response to DNA damage induced by UV irradiation or UV mimetic agents.,similarity:Contains 1 RRM (RNA recognition motif) domain.,tissue specificity:Ubiquitous.,
Catalog No
YM8040
Reactivity
Human, Mouse, Rat
Source
Rabbit IgG, Kappa
Modification
Not Modified
Applications
WB,IHC,IF,IP,ELISA
Dilution
IHC 1:200-1:1000, WB 1:1000-1:5000, IF 1:200-1:1000, ELISA 1:5000-1:20000, IP 1:50-1:200
Purification
Protein A
Storage and Stability
Store at -20°C/1 year
Human Swiss Prot No.
P62736
Gene Name
ACTA2
Protein Name
α smooth muscle actin
Gene ID
59
Other Name
ACTA2;ACTSA;ACTVS;GIG46;Actin; aortic smooth muscle;Alpha-actin-2;Cell growth-inhibiting gene 46 protein
Specificity
Endogenous
Formulation
PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
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Publications (21)
Knockdown of SOX9 alleviates tracheal fibrosis through the Wnt/β-catenin signaling pathway

Author: Available in link provided

Date of Publication : Available in link provided

Ginsenoside Rg2 alleviates myocardial fibrosis by regulating TGF-β1/Smad signalling pathway. PHARMACEUTICAL BIOLOGY Pharm Biol. 2021;59(1):106-113 WB Rat 1:2000 Myocardial

Author: Available in link provided

Date of Publication : Available in link provided

Resveratrol inhibits monocrotaline-induced pulmonary arterial remodeling by suppression of SphK1-mediated NF-κB activation. LIFE SCIENCES Life Sci. 2018 Oct;210:140 WB Rat 1:200 lung PASMCs

Author: Available in link provided

Date of Publication : Available in link provided

PDGF-mediated PI3K/AKT/β-catenin signaling regulates gap junctions in corpus cavernosum smooth muscle cells. EXPERIMENTAL CELL RESEARCH Exp Cell Res. 2018 Jan;362:252 IF Rat 1:100 SMCs

Author: Available in link provided

Date of Publication : Available in link provided

HDAC3 inhibitor suppresses endothelial-to-mesenchymal transition via modulating inflammatory response in atherosclerosis. BIOCHEMICAL PHARMACOLOGY Biochem Pharmacol. 2021 Oct;192:114716 WB Human，Mouse Aortas HUVEC cell

Author: Available in link provided

Date of Publication : Available in link provided

NOGO-B promotes EMT in lung fibrosis via MMP14 mediates free TGF-beta1 formation. Oncotarget Oncotarget. 2017 Sep 19; 8(41): 71024–71037 WB Human 1:1000 A549 cell

Author: Available in link provided

Date of Publication : Available in link provided

SIRT6 inhibits endothelial-to-mesenchymal transition through attenuating the vascular endothelial inflammatory response. INTERNATIONAL IMMUNOPHARMACOLOGY Int Immunopharmacol. 2021 Dec;101:108240 WB Human HUVECs

Author: Available in link provided

Date of Publication : Available in link provided

Huiyang Shengji Extract Improve Chronic Nonhealing Cutaneous through the TGF-β1/Smad3 Signaling Pathway. Evidence-based Complementary and Alternative Medicine Evid-Based Compl Alt. 2021;2021:8881565 WB Human Human skin fibroblasts cells (HSFs)

Author: Available in link provided

Date of Publication : Available in link provided

Lipid accumulation is ahead of epithelial-to-mesenchymal transition and therapeutic intervention by acetyl-CoA carboxylase 2 silence in diabetic nephropathy. METABOLISM-CLINICAL AND EXPERIMENTAL 2014 Feb 27 WB Human HK-2 cell

Author: Available in link provided

Date of Publication : Available in link provided

Salvianolic Acid B-Alleviated Angiotensin II Induces Cardiac Fibrosis by Suppressing NF-κB Pathway In Vitro. MEDICAL SCIENCE MONITOR Med Sci Monitor. 2018; 24: 7654–7664 WB Rat 1: 1000 cardiac fibroblasts (CFBs)

Author: Available in link provided

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Assessing activation of hepatic stellate cells by 99mTc-3PRGD2 scintigraphy targeting integrin αvβ3: a feasibility study. NUCLEAR MEDICINE AND BIOLOGY 2014 Nov 26 WB Rat liver

Author: Available in link provided

Date of Publication : Available in link provided

miR‑221 targets HMGA2 to inhibit bleomycin‑induced pulmonary fibrosis by regulating TGF‑β1/Smad3-induced EMT. INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE 2016 Aug 11 WB Human A549 cell,HBE cell

Author: Available in link provided

Date of Publication : Available in link provided

Epigenetic repression of Krüppel-like factor 4 through Dnmt1 contributes to EMT in renal fibrosis. INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE 2015 Apr 20 WB Human HK-2 cell

Author: Available in link provided

Date of Publication : Available in link provided

Kangfuxin Oral Liquid Attenuates Bleomycin-Induced Pulmonary Fibrosis via the TGF-β1/Smad Pathway. Evidence-based Complementary and Alternative Medicine Evid-Based Compl Alt. 2019;2019:5124026 IHC Rat 1 : 200 Lung tissue

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Date of Publication : Available in link provided

miR-19 targets PTEN and mediates high mobility group protein B1(HMGB1)-induced proliferation and migration of human airway smooth muscle cells. PLoS One 2019 Jun 27 WB Human HASM cell

Author: Available in link provided

Date of Publication : Available in link provided

Immortalized bovine mammary epithelial cells express stem cell markers and differentiate in vitro. CELL BIOLOGY INTERNATIONAL 2016 Jun 19 ICC Human MCF-7 cell

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Date of Publication : Available in link provided

Longitudinal Immune Profiling Highlights CD4+ T Cell Exhaustion Correlated with Liver Fibrosis in Schistosoma japonicum Infection JOURNAL OF IMMUNOLOGY Zhiqiang Qin IF Mouse livers

Author: Available in link provided

Date of Publication : Available in link provided

Regulation of endothelial-to-mesenchymal transition by histone deacetylase 3 posttranslational modifications in neointimal hyperplasia Annals of Translational Medicine Weirong Wang WB Human human umbilical vein endothelial cells (HUVECs)

Author: Available in link provided

Date of Publication : Available in link provided

Cryptotanshinone alleviates lipopolysaccharide and cigarette smoke-induced chronic obstructive pulmonary disease in mice via the Keap1/Nrf2 axis. Jianwen Chen WB，IHC Mouse lung

Author: Available in link provided

Date of Publication : Available in link provided

Inhibición de la Proliferación, Migración e Invasión de Fibroblastos Queloides por miR-183-5p Mediante la Regulación Negativa de EGR1 INTERNATIONAL JOURNAL OF MORPHOLOGY Mingxi Li WB Human 1:1000 Keloid fibroblasts (KFs)

Author: Available in link provided

Date of Publication : Available in link provided

Nobiletin Alleviated Epithelial–Mesenchymal Transition of Hepatocytes in Liver Fibrosis Based on Autophagy—Hippo/YAP Pathway MOLECULAR NUTRITION & FOOD RESEARCH Yuting Hao WB Mouse,Human liver tissue L02 cell

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Primary Antibodies

    • ELISA Kits

    • Research Areas

    Cell Adhesion Proteins Cell Cycle Proteins Channel Proteins Exosome GDP/GTP Binding Proteins Growth Factors and Hormones Homeodomain Proteins Immunohistochemical Kinases and Phosphatases Signaling

     

    Membrane Receptors Neurobiology Signaling Intermediates Steroid Receptors Structural Proteins Synthesis and Degradation Transcription Regulators Transport and Trafficking Tumor Suppressors/Apoptosis
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