actin, alpha 2, smooth muscle, aorta(ACTA2) Homo sapiens. The protein encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Defects in this gene cause aortic aneurysm familial thoracic type 6. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Nov 2008],
Catalog No
N0011
Reactivity
Human,Mouse,Rat,Monkey
Applications
WB,IHC-p,IF/ICC,FCM,ELISA
Modification
Unmodfied/ Total
Source
Monoclonal Mouse
Dilution
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/200 - 1/1000. Immunofluorescence: 1/200 - 1/1000. Flow cytometry: 1/200 - 1/400. ELISA: 1/10000. Not yet tested in other applications.
Immunofluorescence analysis of HepG2 cells using ACTA2 Monoclonal Antibody (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
Immunohistochemistry analysis of paraffin-embedded human duodenum tissues (left) and human esophagus tissues (right) with DAB staining using ACTA2 Monoclonal Antibody.
Western Blot analysis using ACTA2 Monoclonal Antibody against HeLa (1). A431 (2). Jurkat (3). K562 (4). HEK293 (5). HepG2 (6). NIH/3T3 (7). PC-12 (8) and Cos7 (9) cell lysate.
