keratin 18(KRT18)Homo sapiens. KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008],
Catalog No
N0181
Reactivity
Human
Applications
WB,IHC-p,IF(paraffin section),ELISA
Modification
Unmodfied/ Total
Source
Monoclonal Mouse
Dilution
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
Purification
Affinity purification
Concentration
Storage and Stability
-20°C/1 year
Other Name
KRT18; CYK18; PIG46; Keratin; type I cytoskeletal 18; Cell proliferation-inducing gene 46 protein; Cytokeratin-18; CK-18; Keratin-18; K18
Molecular Weight (Da)
Gene Name
KRT18
Protein Name
Keratin type I cytoskeletal 18
Human Gene ID
3875
Human Swiss Prot No.
P05783
Immunogen
Purified recombinant fragment of human Cytokeratin 18 (aa391-483) expressed in E. Coli.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma (A). hepatocarcinoma (B). stomach cancer (C) and colon cancer tissue (D). showing cytoplasmic location with DAB staining using Cytokeratin 18 Monoclonal Antibody.
Western Blot analysis using Cytokeratin 18 Monoclonal Antibody against HeLa (1). NIH/3T3 (2). A549 (3). Jurkat (4). MCF-7(5). HepG2 (6). A431 (7). HEK293 (8) and K562 (9) cell lysate.
