keratin 5(KRT5)Homo sapiens. The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. Mutations in these genes have been associated with a complex of diseases termed epidermolysis bullosa simplex. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. [provided by RefSeq, Jul 2008],
Catalog No
N0190
Reactivity
Human
Applications
WB,IHC-p,IF/ICC,ELISA
Modification
Unmodfied/ Total
Source
Monoclonal Mouse
Dilution
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/200 - 1/1000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
Purification
Affinity purification
Concentration
Storage and Stability
-20°C/1 year
Other Name
KRT5; Keratin; type II cytoskeletal 5; 58 kDa cytokeratin; Cytokeratin-5; CK-5; Keratin-5; K5; Type-II keratin Kb5
Molecular Weight (Da)
Gene Name
KRT5
Protein Name
Keratin type II cytoskeletal 5
Human Gene ID
3852
Human Swiss Prot No.
P13647
Immunogen
Purified recombinant fragment of Cytokeratin 5 expressed in E. Coli.
Immunohistochemistry analysis of paraffin-embedded human lung squamous cell carcinoma (A). normal hepatocyte (B). colon adenocacinoma (C). normal stomach tissue (D). showing cytoplasmic and membrane localization with DAB staining using Cytokeratin (Pan) M
