SMAD family member 5(SMAD5) Homo sapiens. The protein encoded by this gene is involved in the transforming growth factor beta signaling pathway that results in an inhibition of the proliferation of hematopoietic progenitor cells. The encoded protein is activated by bone morphogenetic proteins type 1 receptor kinase, and may be involved in cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014],
Catalog No
N0745
Reactivity
Human,Mouse,Rat,Bovine,Chicken,Pig,sheep
Applications
WB,IF/ICC,FCM
Modification
Unmodfied/ Total
Source
Monoclonal Mouse
Dilution
Western Blot: 1/1000 - 1/2000. Immunofluorescence: 1/100 - 1/500. Flow cytometry: 1/100 - 1/200. Not yet tested in other applications.
Purification
Affinity purification
Concentration
1 mg/ml
Storage and Stability
-20°C/1 year
Other Name
SMAD5; MADH5; Mothers against decapentaplegic homolog 5; MAD homolog 5; Mothers against DPP homolog 5; JV5-1; SMAD family member 5; SMAD 5; Smad5; hSmad5
Molecular Weight (Da)
Gene Name
SMAD5
Protein Name
Mothers against decapentaplegic homolog 5
Human Gene ID
4090
Human Swiss Prot No.
Q99717
Immunogen
Purified recombinant human Smad5 (C-terminus) protein fragments expressed in E.coli.
Specificity
Smad5 Monoclonal Antibody detects endogenous levels of Smad5 protein.
Formulation
Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50% glycerol.
Flow cytometric analysis of Jurkat cells stained with Smad5 Monoclonal Antibody (red). followed by FITC-conjugated goat anti-mouse IgG. Black line histogram represents the isotype control. normal mouse IgG.