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Primary Antibodies
β-actin Monoclonal Antibody(5B7)
β-actin Monoclonal Antibody(5B7)

Assay Biotechnology Inc Antibodies and ELISA Kits have been rigorously validated for laboratory grade quality results. High specificity paired with high affinity for targets of interest give researchers an advantage with our products

Catalog No: N1292
Reactivity:Human,Rat,Mouse,Monkey,Dog,Chicken,Hamster,Rabbit,Insect
Applications:IF/ICC,WB,IHC-p
Modification:Unmodfied/ Total
Source:Monoclonal Mouse
Storage and Stability:-20°C/1 year

$200
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β-actin Monoclonal Antibody(5B7)

Western blot analysis of 1 HEK293 2 SW480 3 HEPG2 4 MCF-7 5 mouse brain 6 Rat brain 7 Hela 8 A549 lysates. primary antibody was diluted at 1:5000. 4° over night. secondary antibody(cat: SA0438 was diluted at 1:10000. 37° 1hour.

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Product Details
Product Name
β-actin Monoclonal Antibody(5B7)
Description
actin beta(ACTB)Homo sapiens. This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq, Jul 2008],
Catalog No
N1292
Reactivity
Human,Rat,Mouse,Monkey,Dog,Chicken,Hamster,Rabbit,Insect
Applications
IF/ICC,WB,IHC-p
Modification
Unmodfied/ Total
Source
Monoclonal Mouse
Dilution
IF: 1:50-200 WB: 1:5000-20000 IHC: 1:200-300
Purification
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Concentration
1 mg/ml
Storage and Stability
-20°C/1 year
Other Name
ACTB; Actin; cytoplasmic 1; Beta-actin; Actin β;β-Actin;Beta actin
Molecular Weight (Da)
41737
Gene Name
ACTB
Protein Name
Actin cytoplasmic 1
Human Gene ID
60
Human Swiss Prot No.
P60709
Immunogen
Synthetic Peptide of β-actin
Specificity
The antibody detects endogenous β-actin protein.
Formulation
PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
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Publications (77)
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Date of Publication : 2022-05-10

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Acute Endoplasmic Reticulum Stress Induces Inflammation Reaction, Complement System Activation, and Lipid Metabolism Disorder of Piglet Livers: A Proteomic Approach

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Date of Publication : 2022-04-13

Irisin Suppresses Nicotine-Mediated Atherosclerosis by Attenuating Endothelial Cell Migration, Proliferation, Cell Cycle Arrest, and Cell Senescence

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Date of Publication : 2022-04-08

N6-methyladenosine modification contributes to arecoline-mediated oral submucosal fibrosis

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Date of Publication : 2022-04-04

Electroacupuncture Alleviates Hyperalgesia by Regulating CB1 Receptor of Spinal Cord in Incisional Neck Pain Rats

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Date of Publication : 2021-12-18

Autophagy and apoptosis mediated nano-copper-induced testicular damage

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Date of Publication : 2021-12-15

Amelioration of Ambient Particulate Matter (PM2.5)-Induced Lung Injury in Rats by Aerobic Exercise Training

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Date of Publication : 2021-10-26

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Protective effects of flavonoids from the leaves of Carya cathayensis Sarg. against H2O2‑induced oxidative damage and apoptosis in vitro

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Tau N-Terminal Inserts Regulate Tau Liquid-Liquid Phase Separation and Condensates Maturation in a Neuronal Cell Model

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Date of Publication : 2021-07-31

Trigeminal neuropathy causes hypomyelination in the anterior cingulate cortex, disrupts the synchrony of neural circuitry, and impairs decision-making in male rats

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Date of Publication : 2021-07-29

Deletion of BK channels decreased skeletal and cardiac muscle function but increased smooth muscle contraction in rats

Author: Chunyu He, Xiaoyue Li, Meili Wang, Suli Zhang, Huirong Liu

Date of Publication : 2021-07-13

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Pten Regulates Cardiomyocyte Differentiation by Modulating Non-CG Methylation via Dnmt3

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Taxifolin inhibits keratinocyte proliferation and ameliorates imiquimod-induced psoriasis-like mouse model via regulating cytoplasmic phospholipase A2 and PPAR-γ pathway

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Date of Publication : 2021-07-04

Gastric cancer-derived exosomal miR-135b-5p impairs the function of Vγ9Vδ2 T cells by targeting specificity protein 1

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Date of Publication : 2021-06-22

Bawei Chenxiang Wan Ameliorates Cardiac Hypertrophy by Activating AMPK/PPAR-α Signaling Pathway Improving Energy Metabolism

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ERK/Drp1‐dependent mitochondrial fission contributes to HMGB1‐induced autophagy in pulmonary arterial hypertension

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Reovirus enhances cytotoxicity of natural killer cells against colorectal cancer via TLR3 pathway

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Activation of AMPK inhibits Galectin-3-induced pulmonary artery smooth muscle cells proliferation by upregulating hippo signaling effector YAP

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7-Difluoromethoxy-5,4′-dimethoxy-genistein attenuates macrophages apoptosis to promote plaque stability via TIPE2/TLR4 axis in high fat diet-fed ApoE−/− mice

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CXCL2 benefits acute myeloid leukemia cells in hypoxia

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Study on the Mechanism of Jiawei Shengjiang Powder in Improving Male Asthma-Induced Asthenospermia Based on Network Pharmacology and Bioinformatics

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Date of Publication : 2021-03-19

Hepatitis B Virus X Protein (HBx) Suppresses Transcription Factor EB (TFEB) Resulting in Stabilization of Integrin Beta 1 (ITGB1) in Hepatocellular Carcinoma Cells

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Effect of leech-centipede medicine on improving erectile function in diabetes-induced erectile dysfunction rats via PDE5 signalling pathway-related molecules

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Date of Publication : 2021-02-11

Dipotassium glycyrrhizinate relieves leptospira-induced nephritis in vitro and in vivo

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Date of Publication : 2021-02-03

Endothelial Agrin Is Dispensable for Normal and Tumor Angiogenesis.

Author: Ye P, Fu Z, Chung JY, Cao X, Ko H, Tian XY, Tang PM, Lui KO

Date of Publication : 2021-01-01

The elevated transcription of ADAM19 by the oncohistone H2BE76K contributes to oncogenic properties in breast cancer

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Date of Publication : 2021-01-01

Nucleotide de novo synthesis increases breast cancer stemness and metastasis via cGMP-PKG-MAPK signaling pathway

Author: Yajing Lv, Xiaoshuang Wang, Xiaoyu Li, Guangwei Xu, Yuting Bai, Jiayi Wu, Yongjun Piao, Yi Shi, Rong Xiang, Longlong Wang

Date of Publication : 2020-11-13

Hypoxia–CXCL6 axis affects arteriolar niche remodeling in acute myeloid leukemia:

Author: Lijun Li, Jiancheng Man, Li Zhao

Date of Publication : 2020-11-09

Effect of leech-centipede medicine on improving erectile function in DIED rats via PKC signalling pathway-related molecules

Author: Ji-Sheng Wang, Xiao Li, Zi-Long Chen, Jun-Long Feng, Bing-Hao Bao, Sheng Deng, Heng-Heng Dai, Fan-Chao Meng, Bin Wang, Hai-Song Li

Date of Publication : 2020-10-10

TNNC1 Reduced Gemcitabine Sensitivity of Nonsmall-Cell Lung Cancer by Increasing Autophagy

Author: Xian Ye, Guanghui Xie, Zhijian Liu, Jun Tang, Mingyuan Cui, Chenbin Wang, Chi Guo, Jianfeng Tang

Date of Publication : 2020-09-18

Biological Network Model of Effect of Chronic Intermittent Hypoxia on Spermatogenesis in Rats

Author: Jisheng Wang, Xuefeng Gong, Fanchao Meng, Sheng Deng, Hengheng Dai, Binghao Bao, Junlong Feng, Haisong Li, Bin Wang

Date of Publication : 2020-09-06

Insulin-like growth factor-1 receptor induces immunosuppression in lung cancer by upregulating B7–H4 expression through the MEK/ERK signaling pathway

Author: Zhiming Zhao, Ningyue Zhang, Anqi Li, Bin Zhou, Yali Chen, Shaomu Chen, Moli Huang, Fengying Wu, Liang Zhang

Date of Publication : 2020-05-14

miR-150-5p mediates extravillous trophoblast cell migration and angiogenesis functions by regulating VEGF and MMP9

Author: Yu Zeng, Lijie Wei, Mwamaka Sharifu Lali, Yuting Chen, Jun Yu, Ling Feng

Date of Publication : 2020-03-05

The Regulatory Effect of the Kinase Inhibitor PD98059 on Autophagic Flux During Trypsinogen Activation in Pancreatic Acinar Cells

Author: Yao Wenchao, Zhu Defu, Lu Haifeng, Liu Chao, Sun Bei, Zhang Weihui, Xue Dongbo

Date of Publication : 2020-02-01

miR-187-5p/apaf-1 axis was involved in oxidative stress-mediated apoptosis caused by ammonia via mitochondrial pathway in chicken livers

Author: Yanmin Xu, Zhuo Li, Shuai Zhang, Hongfu Zhang, Xiaohua Teng

Date of Publication : 2019-12-18

Inhibition of Siah2 ubiquitin ligase ameliorates monocrotaline-induced pulmonary arterial remodeling through inactivation of YAP

Author: Qingting Wang, Wenhua Shi, Qianqian Zhang, Wei Feng, Jian Wang, Cui Zhai, Xin Yan, Manxiang Li

Date of Publication : 2019-12-11

Traditional Chinese Medicine Shenmayizhi Decoction Ameliorates Memory And Cognitive Impairment Induced By Scopolamine Via Preventing Hippocampal Cholinergic Dysfunction In Rats

Author: Qiong Wu, Yu Cao, Meixia Liu, Fang Liu, Adelheid H Brantner, Yang Yang, Yun Wei, Yu Zhou, Zhiyong Wang, Lina Ma, Feixue Wang, Hui Pei, Hao Li

Date of Publication : 2019-11-12

Tanshinone IIA mediates SMAD7-YAP interaction to inhibit liver cancer growth by inactivating the transforming growth factor beta signaling pathway

Author: Lifang Ma, Hongyuan Jiang, Xin Xu, Congcong Zhang, Yongjie Niu, Zhixian Wang, Yuquan Tao, Yan Li, Feng Cai, Xiao Zhang, Xinghe Wang, Yongchun Yu

Date of Publication : 2019-11-11

AMPK activation increases postoperative cognitive impairment in intermittent hypoxia rats via direct activating PAK2

Author: Xi Mei, Guolin Tan, Wenxiang Qing

Date of Publication : 2019-11-07

Adventitial Collagen Crosslink Reduces Intimal Hyperplasia in a Rabbit Arteriovenous Graft Model

Author: Changcheng Liu, Wenyuan Yu, Duanduan Chen, Yue Shi, Zhenfeng Li, Chengxiong Gu

Date of Publication : 2019-10-24

The Effect of SPTLC2 on Promoting Neuronal Apoptosis is Alleviated by MiR-124-3p Through TLR4 Signalling Pathway

Author: Su, Xinhong, Ye, Yuqin, Yang, Yongxiang, Zhang, Kailiang, Bai, Wei, Chen, Huijun, Kang, Enming, Kong, Chuiguang, He, Xiaosheng

Date of Publication : 2019-08-01

Melatonin promotes secondary hair follicle development of early postnatal cashmere goat and improves cashmere quantity and quality by enhancing antioxidant capacity and suppressing apoptosis

Author: Chun‐He Yang, Jian‐Hai Xu, Qing‐Chang Ren, Tao Duan, Fang Mo, Wei Zhang

Date of Publication : 2019-04-03

Ethyl pyruvate protects against Salmonella intestinal infection in mice through down-regulation of pro-inflammatory factors and inhibition of TLR4/MAPK pathway

Author: Na Dong, Xinyao Xu, Chenyu Xue, Chensi Wang, Xinran Li, Anshan Shan, Li Xu, Deshan Li

Date of Publication : 2019-03-19

Neuroprotective effect of Notch pathway inhibitor DAPT against focal cerebral ischemia/reperfusion 3 hours before model establishment

Author: Jun-Jie Wang, Jun-De Zhu, Xian-Hu Zhang, Ting-Ting Long, Guo Ge, Yan Yu

Date of Publication : 2019-03-01

Glycine decarboxylase induces autophagy and is downregulated by miRNA-30d-5p in hepatocellular carcinoma

Author: Zhuang, Hao, Wu, Fei, Wei, Wen, Dang, Yamei, Yang, Baicai, Ma, Xuda, Han, Feng, Li, Yongmei

Date of Publication : 2019-02-25

Glutathione S‑transferase isozyme alpha 1 is predominantly involved in the cisplatin resistance of common types of solid cancer

Author: Mingyue Zou, Xiaolei Hu, Bangtian Xu, Tingting Tong, Yixian Jing, Lei Xi, Wei Zhou, Jinpeng Lu, Xin Wang, Xiaolan Yang, Fei Liao

Date of Publication : 2019-02-01

Facilitating colorectal cancer cell metastasis by targeted binding of long non-coding RNA ENSG00000231881 with miR-133b via VEGFC signaling pathway

Author: 0

Date of Publication : 2018-12-20

The Chinese herbal formula Fuzheng Quxie Decoction attenuates cognitive impairment and protects cerebrovascular function in SAMP8 mice

Author: Feixue Wang, Jianchao Feng, Yang Yang, Jiangang Liu, Meixia Liu, Zhiyong Wang, Hui Pei, Yun Wei, Hao Li

Date of Publication : 2018-11-09

Huaier Augmented the Chemotherapeutic Sensitivity of Oxaliplatin via Downregulation of YAP in Hepatocellular Carcinoma

Author: Yuquan Tao, Liang Shan, Xin Xu, Hongyuan Jiang, Rui Chen, Zijun Qian, Zongguo Yang, Bin Liang, Haiyin Zheng, Feng Cai, Yongchun Yu, Lifang Ma

Date of Publication : 2018-10-10

Aberrant methylation‐induced dysfunction of p16 is associated with osteoblast activation caused by fluoride

Author: Shouli Wu, Weimin Yan, Bing Qiu, Yongfang Liao, Junying Gu, Shaofeng Wei, Aihua Zhang, Xueli Pan

Date of Publication : 2018-09-26

miRNA-23a/CXCR4 regulates neuropathic pain via directly targeting TXNIP/NLRP3 inflammasome axis

Author: Pan, Zhiqiang, Shan, Qun, Gu, Pan, Wang, Xiao Min, Tai, Lydia Wai, Sun, Menglan, Luo, Xin, Sun, Liting, Cheung, Chi Wai

Date of Publication : 2018-01-31

miR-352 participates in the regulation of trypsinogen activation in pancreatic acinar cells by influencing the function of autophagic lysosomes

Author: Zonggong Song, Yongming Huang, Chao Liu, Ming Lu, Zhituo Li, Bei Sun, Weihui Zhang, Dongbo Xue

Date of Publication : 2018-01-13

Huaier Restrains Proliferative and Migratory Potential of Hepatocellular Carcinoma Cells Partially Through Decreased Yes-Associated Protein 1

Author: Liang Shan, Yan Li, Hongyuan Jiang, Yuquan Tao, Zijun Qian, Lan Li, Feng Cai, Lifang Ma, Yongchun Yu

Date of Publication : 2017-11-06

The permeability characteristics and interaction of the main components from Zhizi Bopi decoction in the MDCK cell model

Author: Zhengyue Qian, Cheng Huang, Chenlin Shen, Xiaoming Meng, Zhaolin Chen, Tingting Hu, Yangyang Li, Jun Li

Date of Publication : 2015-12-04

Upregulation of microRNA‑492 induced by epigenetic drug treatment inhibits the malignant phenotype of clear cell renal cell carcinoma in vitro

Author: Aibing Wu, Kunpeng Wu, Mingchun Li, Lingli Bao, Xiang Shen, Shunjun Li, Jinmei Li, Zhixiong Yang

Date of Publication : 2015-03-26

The Regenerating Spinal Cord of Gecko Maintains Unaltered Expression of β-Catenin Following Tail Amputation

Author: Song, Honghua, Man, Lili, Wang, Yingjie, Bai, Xue, Wei, Sumei, Liu, Yan, Liu, Mei, Gu, Xiaosong, Wang, Yongjun

Date of Publication : 2014-09-02

Extracts of Dracocephalum tanguticum Maxim Ameliorate Acute Alcoholic Liver Disease via Regulating Transcription Factors in Mice.

Author: Min Guo, Liwei Gu, Heping Hui, Xiaodong Li，Ling Jin

Date of Publication : 44636

Co-exposure of polystyrene microplastics and iron aggravates cognitive decline in aging mice via ferroptosis induction

Author: Xiu Liu, Hekai Yang, Xinzhu Yan, Shuangfeng Xu, Yonggang Fan, He Xu, Yue Ma, Weijian Hou, Rabia Javed, Yanhui Zhang

Date of Publication : 44617

DJ-1 upregulates the Nrf2/GPX4 signal pathway to inhibit trophoblast ferroptosis in the pathogenesis of preeclampsia

Author: Liao, Tingting, Xu, Xia, Ye, Xu, Yan, Jianying

Date of Publication : 44613

Exosomal miRNA-328-3p targets ZO-3 and inhibits porcine epidemic diarrhea virus proliferation

Author: Zhao, Han, Yang, Jinxin, Wang, Qian, Cui, Zhanding, Li, Dengliang, Niu, Jiangting, Guo, Yanbing, Zhang, Qian, Zhang, Shuang, Zhao, Yanli, Wang, Kai, Lian, Wei, Hu, Guixue

Date of Publication : 44603

Primary Antibodies

    • ELISA Kits

    • Research Areas

    Cell Adhesion Proteins Cell Cycle Proteins Channel Proteins Exosome GDP/GTP Binding Proteins Growth Factors and Hormones Homeodomain Proteins Immunohistochemical Kinases and Phosphatases Signaling

     

    Membrane Receptors Neurobiology Signaling Intermediates Steroid Receptors Structural Proteins Synthesis and Degradation Transcription Regulators Transport and Trafficking Tumor Suppressors/Apoptosis
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