cytochrome c oxidase subunit 4I1(COX4I1)Homo sapiens. Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit IV isoform 1 of the human mitochondrial respiratory chain enzyme. It is located at the 3' of the NOC4 (neighbor of COX4) gene in a head-to-head orientation, and shares a promoter with it. Pseudogenes related to this gene are located on chromosomes
Catalog No
N1297
Reactivity
Human,Rat,Mouse
Applications
WB,IHC-p,IF/ICC,
Modification
Unmodfied/ Total
Source
Monoclonal Mouse
Dilution
WB: 1:1000-3000 IF 1:200 IHC 1:50-300
Purification
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Concentration
Storage and Stability
-20°C/1 year
Other Name
COX4I1; COX4; Cytochrome c oxidase subunit 4 isoform 1, mitochondrial; Cytochrome c oxidase polypeptide IV; Cytochrome c oxidase subunit IV isoform 1; COX IV-1
Molecular Weight (Da)
19577
Gene Name
COX4I1
Protein Name
Cytochrome c oxidase subunit 4 isoform 1, mitochondrial
Human Gene ID
1327
Human Swiss Prot No.
P13073
Immunogen
Recombinant Protein of Cytochrome c oxidase subunit 4 isoform 1, mitochondrial
Specificity
The antibody detects endogenous COX IV protein.
Formulation
PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
Immunohistochemical analysis of paraffin-embedded Mouse-colon tissue. 1.COX IV Monoclonal Antibody(6C8) was diluted at 1:200(4°C.overnight). 2. Sodium citrate pH 6.0 was used for antibody retrieval(>98°C.20min). 3.Secondary antibody was diluted at 1:200(room temperature. 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1.COX IV Monoclonal Antibody(6C8) was diluted at 1:200(4°C.overnight). 2. Sodium citrate pH 6.0 was used for antibody retrieval(>98°C.20min). 3.Secondary antibody was diluted at 1:200(room temperature. 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Human-uterus-cancer tissue. 1.COX IV Monoclonal Antibody(6C8) was diluted at 1:200(4°C.overnight). 2. Sodium citrate pH 6.0 was used for antibody retrieval(>98°C.20min). 3.Secondary antibody was diluted at 1:200(room temperature. 30min). Negative control was used by secondary antibody only.
Western blot analysis of lysates from 1) COS7.2) 3T3.3) Hela cells. (Green) primary antibody was diluted at 1:1000. 4°over night. Dylight 800 secondary antibody(Assay Biotech:SA0438)was diluted at 1:10000. 37° 1hour. (Red) Actin β Polyclonal Antibody (Assay Biotech:C40022) antibody was diluted at 1:5000 as loading control. 4° over night.Dylight 680 secondary antibody(Assay Biotech:SA0437)was diluted at 1:10000. 37° 1hour.
Immunofluorescence analysis of Hela cell. 1.AF-10 Polyclonal Antibody(red) was diluted at 1:200(4° overnight). COX IV Monoclonal Antibody(6C8)(green) was diluted at 1:200(4° overnight). 2. Goat Anti Rabbit Alexa Fluor 594 Catalog:SA0837 was diluted at 1:1000(room temperature. 50min). Goat Anti Mouse Alexa Fluor 488 Catalog:SA0662 was diluted at 1:1000(room temperature. 50min).
Zhang. Yanqiong. et al. "A discovery of clinically approved formula FBRP for repositioning to treat HCC by inhibiting PI3K/AKT/NF-κB activation." Molecular Therapy-Nucleic Acids19 (2020): 890-904.
