tubulin alpha 1a(TUBA1A)Homo sapiens. Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. The genes encoding these microtubule constituents belong to the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species. This gene encodes alpha tubulin and is highly similar to the mouse and rat Tuba1 genes. Northern blotting studies have shown that the gene expression is predominantly found in morphologically differentiated neurologic cells. This gene is one of three alpha-tubulin genes in a cluster on chromosome 12q.
Catalog No
N1299
Reactivity
Human,Rat,Mouse
Applications
WB,IHC-p,IF/ICC,IP
Modification
Unmodfied/ Total
Source
Monoclonal Mouse
Dilution
WB: 1:500-10000 IP:1:200 IF 1:200 IHC 1:50-300
Purification
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Concentration
Storage and Stability
-20°C/1 year
Other Name
Molecular Weight (Da)
50136
Gene Name
TUBA1A
Protein Name
Tubulin alpha-1A chain
Human Gene ID
7846/10376
Human Swiss Prot No.
Q71U36/P68363
Immunogen
Recombinant Protein of Tubulin alpha-1A chain
Specificity
The antibody detects endogenousα-tubulin protein.
Formulation
PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
Immunohistochemical analysis of paraffin-embedded Mouse-heart tissue. 1.α-tubulin Monoclonal Antibody(8F11) was diluted at 1:200(4°C.overnight). 2. Sodium citrate pH 6.0 was used for antibody retrieval(>98°C.20min). 3.Secondary antibody was diluted at 1:200(room temperature. 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1.α-tubulin Monoclonal Antibody(8F11) was diluted at 1:200(4°C.overnight). 2. Sodium citrate pH 6.0 was used for antibody retrieval(>98°C.20min). 3.Secondary antibody was diluted at 1:200(room temperature. 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Human-uterus-cancer tissue. 1.α-tubulin Monoclonal Antibody(8F11) was diluted at 1:200(4°C.overnight). 2. Sodium citrate pH 6.0 was used for antibody retrieval(>98°C.20min). 3.Secondary antibody was diluted at 1:200(room temperature. 30min). Negative control was used by secondary antibody only.
Immunofluorescence analysis of Hela cell. 1.DAPK3 (phospho Thr265) Polyclonal Antibody(red) was diluted at 1:200(4° overnight). α-tubulin Monoclonal Antibody(8F11)(green) was diluted at 1:200(4° overnight). 2. Goat Anti Rabbit Alexa Fluor 594 Catalog:SA0837 was diluted at 1:1000(room temperature. 50min). Goat Anti Mouse Alexa Fluor 488 Catalog:SA0662 was diluted at 1:1000(room temperature. 50min).
Wang. T.. Liang. L.. Zhao. C. et al. Elucidating direct kinase targets of compound Danshen dropping pills employing archived data and prediction models. Sci Rep 11. 9541 (2021).
