keratin 8(KRT8)Homo sapiens. This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2012],
Catalog No
N1320
Reactivity
Human,Mouse,Rat
Applications
IF/ICC,WB,IHC-p
Modification
Unmodfied/ Total
Source
Monoclonal Mouse
Dilution
IF: 1:50-200 WB: 1:2000-5000 IHC: 1:200
Purification
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Concentration
Storage and Stability
-20°C/1 year
Other Name
KRT8; CYK8; Keratin, type II cytoskeletal 8; Cytokeratin-8; CK-8; Keratin-8; K8; Type-II keratin Kb8
Molecular Weight (Da)
53573
Gene Name
KRT8
Protein Name
Keratin type II cytoskeletal 8
Human Gene ID
3856
Human Swiss Prot No.
P05787
Immunogen
Synthetic Peptide of CK8
Specificity
The antibody detects endogenous CK8 proteins.
Formulation
PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
Immunohistochemical analysis of paraffin-embedded Mouse-liver tissue. 1.CK8 Monoclonal Antibody(8G8) was diluted at 1:200(4°C.overnight). 2. Sodium citrate pH 6.0 was used for antibody retrieval(>98°C.20min). 3.Secondary antibody was diluted at 1:200(room temperature. 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Rat-liver tissue. 1.CK8 Monoclonal Antibody(8G8) was diluted at 1:200(4°C.overnight). 2. Sodium citrate pH 6.0 was used for antibody retrieval(>98°C.20min). 3.Secondary antibody was diluted at 1:200(room temperature. 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Human-colon tissue. 1.CK8 Monoclonal Antibody(8G8) was diluted at 1:200(4°C.overnight). 2. Sodium citrate pH 6.0 was used for antibody retrieval(>98°C.20min). 3.Secondary antibody was diluted at 1:200(room temperature. 30min). Negative control was used by secondary antibody only.
