keratin 16(KRT16)Homo sapiens. The protein encoded by this gene is a member of the keratin gene family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Most of the type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains and are clustered in a region of chromosome 17q12-q21. This keratin has been coexpressed with keratin 14 in a number of epithelial tissues, including esophagus, tongue, and hair follicles. Mutations in this gene are associated with type 1 pachyonychia congenita, non-epidermolytic palmoplantar keratoderma and unilateral palmoplantar verrucous nevus. [provided by RefSeq, Jul 2008],
Catalog No
N1321
Reactivity
Human,Mouse,Rat
Applications
IHC-P,IF/ICC
Modification
Unmodfied/ Total
Source
Monoclonal Mouse
Dilution
WB 500-2000 1:200 IF 1:50-200
Purification
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Concentration
Storage and Stability
-20°C/1 year
Other Name
KRT16; KRT16A; Keratin, type I cytoskeletal 16; Cytokeratin-16; CK-16; Keratin-16; K16
Molecular Weight (Da)
51268
Gene Name
KRT16
Protein Name
Keratin type I cytoskeletal 16
Human Gene ID
3868
Human Swiss Prot No.
P08779
Immunogen
Synthetic Peptide of CK16
Specificity
The antibody detects endogenous CK16 proteins.
Formulation
PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1.CK16 Monoclonal Antibody(6F6) was diluted at 1:200(4°C.overnight). 2. Sodium citrate pH 6.0 was used for antibody retrieval(>98°C.20min). 3.Secondary antibody was diluted at 1:200(room temperature. 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Human-Tonsil tissue. 1.CK16 Monoclonal Antibody(6F6) was diluted at 1:200(4°C.overnight). 2. Sodium citrate pH 6.0 was used for antibody retrieval(>98°C.20min). 3.Secondary antibody was diluted at 1:200(room temperature. 30min). Negative control was used by secondary antibody only.
