caudal type homeobox 2(CDX2) Homo sapiens. This gene is a member of the caudal-related homeobox transcription factor gene family. The encoded protein is a major regulator of intestine-specific genes involved in cell growth an differentiation. This protein also plays a role in early embryonic development of the intestinal tract. Aberrant expression of this gene is associated with intestinal inflammation and tumorigenesis. [provided by RefSeq, Jan 2012],
Catalog No
N1322
Reactivity
Human,Mouse,Rat
Applications
WB,IF/ICC,IHC-p
Modification
Unmodfied/ Total
Source
Monoclonal Mouse
Dilution
WB: 1:1000 IHC: 1:200 IF 1:200
Purification
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Concentration
Storage and Stability
-20°C/1 year
Other Name
CDX2; CDX3; Homeobox protein CDX-2; CDX-3; Caudal-type homeobox protein 2
Molecular Weight (Da)
33510
Gene Name
CDX2
Protein Name
Homeobox protein CDX-2
Human Gene ID
1045
Human Swiss Prot No.
Q99626
Immunogen
Synthetic Peptide of CDX2
Specificity
The antibody detects endogenous CDX2 proteins.
Formulation
PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
Western blot analysis of 1) 293T. 2) Mouse Heart tissue. diluted at 1:2000. cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003.Inventbiotech.MN.USA).
Immunohistochemical analysis of paraffin-embedded Mouse-testis tissue. 1.CDX2 Monoclonal Antibody(14H6) was diluted at 1:200(4°C.overnight). 2. Sodium citrate pH 6.0 was used for antibody retrieval(>98°C.20min). 3.Secondary antibody was diluted at 1:200(room temperature. 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1.CDX2 Monoclonal Antibody(14H6) was diluted at 1:200(4°C.overnight). 2. Sodium citrate pH 6.0 was used for antibody retrieval(>98°C.20min). 3.Secondary antibody was diluted at 1:200(room temperature. 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Human-colon tissue. 1.CDX2 Monoclonal Antibody(14H6) was diluted at 1:200(4°C.overnight). 2. Sodium citrate pH 6.0 was used for antibody retrieval(>98°C.20min). 3.Secondary antibody was diluted at 1:200(room temperature. 30min). Negative control was used by secondary antibody only.
Immunofluorescence analysis of Hela cell. 1.Amyloid-β Polyclonal Antibody(green) was diluted at 1:200(4° overnight). (red) was diluted at 1:200(4° overnight). 2. Goat Anti Rabbit Alexa Fluor 488 Catalog:SA0665 was diluted at 1:1000(room temperature. 50min). Goat Anti Mouse Alexa Fluor 594 Catalog:SA0834 was diluted at 1:1000(room temperature. 50min).
