carbonic anhydrase 9(CA9)Homo sapiens. Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA IX is a transmembrane protein and is one of only two tumor-associated carbonic anhydrase isoenzymes known. It is expressed in all clear-cell renal cell carcinoma, but is not detected in normal kidney or most other normal tissues. It may be involved in cell proliferation and transformation. This gene was mapped to 17q21.2 by fluorescence in situ hybridization, however, radiation hybrid mapping localized it to 9p13-p12. [provided by RefSeq, Jun 2014],
Catalog No
N1341
Reactivity
Human
Applications
IF/ICC,WB,IHC-p,IP
Modification
Unmodfied/ Total
Source
Monoclonal Mouse
Dilution
IF: 1:50-200 WB: 1:3000 IP:1:200 IHC 1:50-300
Purification
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Immunohistochemical analysis of paraffin-embedded Human stomach. 1. Antibody was diluted at 1:100(4° overnight). 2. High-pressure and temperature EDTA. pH8.0 was used for antigen retrieval. 3.Secondary antibody was diluted at 1:200(room temperature. 30min).
Immunohistochemical analysis of paraffin-embedded Human stomach. 1. Antibody was diluted at 1:100(4° overnight). 2. High-pressure and temperature EDTA. pH8.0 was used for antigen retrieval. 3.Secondary antibody was diluted at 1:200(room temperature. 30min).
Immunohistochemical analysis of paraffin-embedded Human stomach. 1. Antibody was diluted at 1:100(4° overnight). 2. High-pressure and temperature EDTA. pH8.0 was used for antigen retrieval. 3.Secondary antibody was diluted at 1:200(room temperature. 30min).
Immunohistochemical analysis of paraffin-embedded Human stomach. 1. Antibody was diluted at 1:100(4° overnight). 2. High-pressure and temperature EDTA. pH8.0 was used for antigen retrieval. 3.Secondary antibody was diluted at 1:200(room temperature. 30min).
Immunohistochemical analysis of paraffin-embedded Human gallbladder. 1. Antibody was diluted at 1:100(4° overnight). 2. High-pressure and temperature EDTA. pH8.0 was used for antigen retrieval. 3.Secondary antibody was diluted at 1:200(room temperature. 30min).
Immunohistochemical analysis of paraffin-embedded Human gallbladder. 1. Antibody was diluted at 1:100(4° overnight). 2. High-pressure and temperature EDTA. pH8.0 was used for antigen retrieval. 3.Secondary antibody was diluted at 1:200(room temperature. 30min).
Immunohistochemical analysis of paraffin-embedded Human gallbladder. 1. Antibody was diluted at 1:100(4° overnight). 2. High-pressure and temperature EDTA. pH8.0 was used for antigen retrieval. 3.Secondary antibody was diluted at 1:200(room temperature. 30min).
Western blot analysis of 1) Hela. 2) 293T. diluted at 1:5000. cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003.Inventbiotech.MN.USA).
Immunohistochemical analysis of paraffin-embedded Human-lung-cancer tissue. 1.CA IX Monoclonal Antibody(12F10) was diluted at 1:200(4°C.overnight). 2. Sodium citrate pH 6.0 was used for antibody retrieval(>98°C.20min). 3.Secondary antibody was diluted at 1:200(room temperature. 30min). Negative control was used by secondary antibody only.
Western blot analysis of lysates from 1) Hela. 2) 293T cells. (Green) primary antibody was diluted at 1:1000. 4°over night. Dylight 800 secondary antibody(Assay Biotech:SA0438)was diluted at 1:10000. 37° 1hour. (Red) Tubulin β Polyclonal Antibody (Assay Biotech:C0380) antibody was diluted at 1:5000 as loading control. 4° over night.Dylight 680 secondary antibody(Assay Biotech:SA0437)was diluted at 1:10000. 37° 1hour.
