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Primary Antibodies
NFkB p65 Monoclonal Antibody(5G6)
NFkB p65 Monoclonal Antibody(5G6)

Assay Biotechnology Inc Antibodies and ELISA Kits have been rigorously validated for laboratory grade quality results. High specificity paired with high affinity for targets of interest give researchers an advantage with our products

Catalog No: N1376
Reactivity:Human,Mouse,Rat
Applications:WB,IHC-p,IF/ICCIP
Modification:Unmodfied/ Total
Source:Monoclonal Mouse
Storage and Stability:-20°C/1 year

$160
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NFkB p65 Monoclonal Antibody(5G6)

Western blot analysis of 1) Hela. 2) Rat Heart Tissue. 3) Mouse Spleen Tissue. diluted at 1:2000.

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Product Details
Product Name
NFkB p65 Monoclonal Antibody(5G6)
Description
NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011],
Catalog No
N1376
Reactivity
Human,Mouse,Rat
Applications
WB,IHC-p,IF/ICC,IP
Modification
Unmodfied/ Total
Source
Monoclonal Mouse
Dilution
WB: 1:1000-3000 IP:1:200 IF 1:200 IHC 1:50-300
Purification
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Concentration
Storage and Stability
-20°C/1 year
Other Name
RELA; NFKB3; Transcription factor p65; Nuclear factor NF-kappa-B p65 subunit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3
Molecular Weight (Da)
60219
Gene Name
RELA
Protein Name
Transcription factor p65
Human Gene ID
5970
Human Swiss Prot No.
Q04206
Immunogen
Recombinant Protein of Transcription factor p65
Specificity
The antibody detects endogenous p65 proteins.
Formulation
PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
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Publications (46)
Preliminary verification of the anti-hypoxia mechanism of Gentiana straminea maxim based on UPLC-triple TOF MS/MS and network pharmacology

Author: Available in link provided

Date of Publication : Available in link provided

STING signaling sensing of DRP1-dependent mtDNA release in kupffer cells contributes to lipopolysaccharide-induced liver injury in mice WB Mouse 1:1000 /KCs, hepatocytes

Author: Available in link provided

Date of Publication : Available in link provided

Imbalance of alveolar epithelial type I and type Ⅱ cells in lipopolysaccharide-induced chronic lung injury young mouse model WB Mouse 1:1000 Lung/

Author: Available in link provided

Date of Publication : Available in link provided

Bacillus subtilis ameliorates Escherichia coli-induced endometritis in mice via maintaining endometrial barrier and inhibiting inflammatory response. MICROBIAL PATHOGENESIS2022 May;166:105487. Mouse uterine tissue

Author: Available in link provided

Date of Publication : Available in link provided

Mechanical loading mitigates osteoarthritis symptoms by regulating the inflammatory microenvironment in a mouse model 2022 Feb 27. WB,IHC Mouse 1:100 articular cartilage

Author: Available in link provided

Date of Publication : Available in link provided

Overexpression of angiotensin-converting enzyme 2 contributes to the amelioration of Streptococcus uberis-induced inflammatory injury in mammary epithelial cells Vet Microbiol. 2022 May;268:109398. WB Mouse

Author: Available in link provided

Date of Publication : Available in link provided

Electroacupuncture Prevents the Depression-Like Behavior by Inhibiting the NF-κB/NLRP3 Inflammatory Pathway in Hippocampus of Mice Subjected to Chronic Mild Stress Neuropsychobiology. ;:1-9. WB Mouse 1:1000 hippocampus

Author: Available in link provided

Date of Publication : Available in link provided

Selected lactobacilli strains inhibit inflammation in LPS-induced RAW264.7 macrophages by suppressing the TLR4-mediated NF-κB and MAPKs activation Food Sci Tech-Brazil. 2022 Mar;42:. WB Mouse 1:500

Author: Available in link provided

Date of Publication : Available in link provided

Arsenic trioxide induces expression of BCL-2 expression via NF-κB and p38 MAPK signaling pathways in BEAS-2B cells during apoptosis. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY Ecotox Environ Safe. 2021 Oct;222:112531 IF Human 1:100 BEAS-2B cell

Author: Available in link provided

Date of Publication : Available in link provided

Ganoderic acid alleviates chemotherapy-induced fatigue in mice bearing colon tumor. ACTA PHARMACOLOGICA SINICA Acta Pharmacol Sin. 2021 Apr;42(10):1703-1713 WB Mouse 1 : 1000 Skeletal muscles，hippocampi

Author: Available in link provided

Date of Publication : Available in link provided

The Role and Mechanism of SIRT1 in Resveratrol-regulated Osteoblast Autophagy in Osteoporosis Rats. Scientific Reports Sci Rep-Uk. 2019 Dec;9(1):1-15 WB Mouse osteoblasts

Author: Available in link provided

Date of Publication : Available in link provided

The Role and Mechanism of SIRT1 in Resveratrol-regulated Osteoblast Autophagy in Osteoporosis Rats. Scientific Reports Sci Rep-Uk. 2019 Dec;9(1):1-15 WB Mouse osteoblasts

Author: Available in link provided

Date of Publication : Available in link provided

Sucralose enhances the susceptibility to dextran sulfate sodium (DSS) induced colitis in mice with changes in gut microbiota. Food & Function Food Funct. 2021 Oct;12(19):9380-9390 WB Mouse Colonic tissue

Author: Available in link provided

Date of Publication : Available in link provided

17β-Estradiol alleviates intervertebral disc degeneration by inhibiting NF-κB signal pathway. LIFE SCIENCES Life Sci. 2021 Nov;284:119874 IHC Rat 1:100 Interverbal disc (IVD)

Author: Available in link provided

Date of Publication : Available in link provided

The Protective Effects of Lactobacillus plantarum KLDS 1.0344 on LPS-Induced Mastitis In Vitro and In Vivo. Frontiers in Immunology Front Immunol. 2021; 12: 770822 WB Mouse 1:500 Breast

Author: Available in link provided

Date of Publication : Available in link provided

DCLK1 promotes epithelial‐mesenchymal transition via the PI3K/Akt/NF‐κB pathway in colorectal cancer. INTERNATIONAL JOURNAL OF CANCER Int J Cancer. 2018 May;142(10):2068-2079 IF Human 1:200 HCT116 cell ,SW480 cell

Author: Available in link provided

Date of Publication : Available in link provided

Salmonella Effector SpvB Inhibits NF-κB Activity via KEAP1-Mediated Downregulation of IKKβ. Frontiers in Cellular and Infection Microbiology Front Cell Infect Mi. 2021 Mar;11:207 WB Human HeLa cells，293T cells

Author: Available in link provided

Date of Publication : Available in link provided

Aurantio-obtusin induces hepatotoxicity through activation of NLRP3 inflammasome signaling. TOXICOLOGY LETTERS Toxicol Lett. 2022 Jan;354:1 WB Mouse 1:500 Liver

Author: Available in link provided

Date of Publication : Available in link provided

Suppression of CDCA3 inhibits prostate cancer progression via NF‑κB/cyclin D1 signaling inactivation and p21 accumulation. ONCOLOGY REPORTS Oncol Rep. 2022 Feb;47(2):1-10 WB Human 1:1000 Prostate cancer tissue,paracancerous tissue.

Author: Available in link provided

Date of Publication : Available in link provided

Aerobic exercise ameliorates particulate matter-induced lung injury in aging rats. ENVIRONMENTAL POLLUTION Environ Pollut. 2021 Mar;:116889 WB Rat 1:2000 Lung

Author: Available in link provided

Date of Publication : Available in link provided

5-Lipoxygenase inhibition reduces inflammation and neuronal apoptosis via AKT signaling after subarachnoid hemorrhage in rats. Aging-US Aging-Us. 2021 Apr 30; 13(8): 11752–11761 WB Rat 1 : 1000 Brain

Author: Available in link provided

Date of Publication : Available in link provided

AMBMP activates WNT pathway and alleviates stress-induced behaviors in maternal separation and chronic stress models. EUROPEAN JOURNAL OF PHARMACOLOGY Eur J Pharmacol. 2020 Aug;881:173192 WB Mouse 1:500 Hippocampal tissue

Author: Available in link provided

Date of Publication : Available in link provided

Peptides derived from rice α-globulin reduce atherosclerosis in apolipoprotein E-deficient mice by inhibiting TNF-α-induced vascular endothelial cells injury. Journal of Functional Foods 2019 Sep 20 WB Human 1:2000 HUVECs

Author: Available in link provided

Date of Publication : Available in link provided

Influence of the total saponin fraction from Dioscorea nipponica Makino on TLR2/4-IL1R receptor singnal pathway in rats of gouty arthritis. JOURNAL OF ETHNOPHARMACOLOGY J Ethnopharmacol. 2017 Jul;206:274 WB Rat 1: 2000 sunovial tissue

Author: Available in link provided

Date of Publication : Available in link provided

Zhibaidihuang Decoction Ameliorates Cell Oxidative Stress by Regulating the Keap1-Nrf2-ARE Signalling Pathway. Evidence-based Complementary and Alternative Medicine Evid-Based Compl Alt. 2020;2020:9294605 WB,IHC Rat 1 : 200 Oral mucosa

Author: Available in link provided

Date of Publication : Available in link provided

Aβ40 Promotes the Osteoblastic Differentiation of Aortic Valve Interstitial Cells through the RAGE Pathway. Current Medical Science 2020 Oct 29 WB Human 1:1000 Acute aortic aortic valve interstitial cells (AVICs)

Author: Available in link provided

Date of Publication : Available in link provided

Hu, Yusheng, et al. "Protective Effect of Kushenlu on Lipopolysaccharide-induced Small Intestinal Inflammation in Rats." International Journal of Pharmacology 13.5 (2017): 473-480.

Author: Available in link provided

Date of Publication : Available in link provided

Thomson, I. S. I. "Yusheng Hu, Ruying Lin, Yifei Bian, Kai Fan and Zhongjie Liu." International Journal of Pharmacology 13.5 (2017): 473-480.

Author: Available in link provided

Date of Publication : Available in link provided

Chidamide Combined With Doxorubicin Induced p53-Driven Cell Cycle Arrest and Cell Apoptosis Reverse Multidrug Resistance of Breast Cancer. Frontiers in Oncology Front Oncol. 2021 Mar;0:344

Author: Available in link provided

Date of Publication : Available in link provided

Establishment and Mechanism Study of a Primary Ovarian Insufficiency Mouse Model Using Lipopolysaccharide. Analytical Cellular Pathology Anal Cell Pathol. 2021;2021:1781532 WB Mouse 1:1000 Ovarian tissue

Author: Available in link provided

Date of Publication : Available in link provided

LXA4 ameliorates cerebrovascular endothelial dysfunction by reducing acute inflammation after subarachnoid hemorrhage in rats. NEUROSCIENCE Neuroscience. 2019 Jun;408:105 WB Rat 1:1000 brain

Author: Available in link provided

Date of Publication : Available in link provided

Therapeutic Potential of Liraglutide for Diabetes–Periodontitis Comorbidity: Killing Two Birds with One Stone Journal of Diabetes Research Rongbing Tang IHC Rat

Author: Available in link provided

Date of Publication : Available in link provided

TcpC Inhibits M1 but Promotes M2 Macrophage Polarization via Regulation of the MAPK/NF-κB and Akt/STAT6 Pathways in Urinary Tract Infection Cells Jianping Pan WB Human,Mouse

Author: Available in link provided

Date of Publication : Available in link provided

Functional and metabolic alterations of gut microbiota in children with new-onset type 1 diabetes

Author: Available in link provided

Date of Publication : Available in link provided

Protective Effect of Lactiplantibacillus plantarum subsp. plantarum SC-5 on Dextran Sulfate Sodium—Induced Colitis in Mice Foods Pan Hu WB Mouse Colon tissue

Author: Available in link provided

Date of Publication : Available in link provided

Euphohelides A–C, ent-Abietane-Type Norditerpene Lactones from Euphorbia helioscopia and Their Anti-Inflammatory Activities JOURNAL OF NATURAL PRODUCTS Kun Gao WB Mouse RAW264.7 cell

Author: Available in link provided

Date of Publication : Available in link provided

Coelonin protects against PM2.5-induced macrophage damage via suppressing TLR4/NF-κB/COX-2 signaling pathway and NLRP3 inflammasome activation in vitro ENVIRONMENTAL TOXICOLOGY Zhi-Shan Ding IF Mouse RAW264.7 cell,J774A.1 cell

Author: Available in link provided

Date of Publication : Available in link provided

Dangshen Huangjiu exerts prevention of gastric mucosal injury and inhibits Akt/NF-κB signaling. Fangdi Hu WB Rat,Human gastric mucosal tissues GES-1 cell

Author: Available in link provided

Date of Publication : Available in link provided

L-Theanine delays D-galactose-induced senescence by regulating the cell cycle and inhibiting apoptosis in rat intestinal cells JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE Wei Xu WB Rat 1:2000 IEC-6 cell

Author: Available in link provided

Date of Publication : Available in link provided

Catalpol rescues LPS-induced cognitive impairment via inhibition of NF-Κb-regulated neuroinflammation and up-regulation of TrkB-mediated BDNF secretion in mice JOURNAL OF ETHNOPHARMACOLOGY Weiqing Hu WB Mouse 1:1000 BV2 cell

Author: Available in link provided

Date of Publication : Available in link provided

Thermosensitive hydrogel with emodin-loaded triple-targeted nanoparticles for a rectal drug delivery system in the treatment of chronic non-bacterial prostatitis JOURNAL OF NANOBIOTECHNOLOGY Ye Yan WB Rat,Mouse prostate tissue RAW 264.7 cell

Author: Available in link provided

Date of Publication : Available in link provided

Mdivi-1 alleviates sepsis-induced liver injury by inhibiting STING signaling activation SHOCK Zhang Qin WB Mouse 1:1000 kupffer cells (KCs)

Author: Available in link provided

Date of Publication : Available in link provided

Polystyrene nanoplastics induce intestinal and hepatic inflammation through activation of NF-κB/NLRP3 pathways and related gut-liver axis in mice SCIENCE OF THE TOTAL ENVIRONMENT Xuanwei Chen WB Mouse 1:1000 intestinal tissue,liver tissue

Author: Available in link provided

Date of Publication : Available in link provided

Phillyrin reduces ROS production to alleviate the progression of intervertebral disc degeneration by inhibiting NF-κB pathway Journal of Orthopaedic Surgery and Research Chen Enming WB Rat 1:1000 nucleus pulposus cell (NPC)

Author: Available in link provided

Date of Publication : Available in link provided

Mitochondria-targeted drug delivery system based on tetrahedral framework nucleic acids for bone regeneration under oxidative stress CHEMICAL ENGINEERING JOURNAL Yanjing Li WB Rat Primary periodontal stem cell(PDLSC)

Author: Available in link provided

Date of Publication : Available in link provided

α-amanitin induce inflammatory response by activating ROS/NF-κB-NLRP3 signaling pathway in human hepatoma HepG2 cells CHEMOSPHERE Zhiyong Zhao WB Human HepG2 cell

Author: Available in link provided

Date of Publication : Available in link provided

Primary Antibodies

    • ELISA Kits

    • Research Areas

    Cell Adhesion Proteins Cell Cycle Proteins Channel Proteins Exosome GDP/GTP Binding Proteins Growth Factors and Hormones Homeodomain Proteins Immunohistochemical Kinases and Phosphatases Signaling

     

    Membrane Receptors Neurobiology Signaling Intermediates Steroid Receptors Structural Proteins Synthesis and Degradation Transcription Regulators Transport and Trafficking Tumor Suppressors/Apoptosis
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