actin, alpha 1, skeletal muscle(ACTA1) Homo sapiens. The product encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Mutations in this gene cause nemaline myopathy type 3, congenital myopathy with excess of thin myofilaments, congenital myopathy with cores, and congenital myopathy with fiber-type disproportion, diseases that lead to muscle fiber defects. [provided by RefSeq, Jul 2008],
Catalog No
N1414
Reactivity
Human,Mouse,Rat
Applications
WB,IHC-p,IF/ICC,IP
Modification
Unmodfied/ Total
Source
Monoclonal Mouse
Dilution
WB: 1:500-10000 IP:1:200 IF 1:200 IHC 1:50-300
Purification
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Immunohistochemical analysis of paraffin-embedded Mouse-liver tissue. 1.α skeletal muscle actin Monoclonal Antibody(4B11) was diluted at 1:200(4°C.overnight). 2. Sodium citrate pH 6.0 was used for antibody retrieval(>98°C.20min). 3.Secondary antibody was diluted at 1:200(room temperature. 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Rat-liver tissue. 1.α skeletal muscle actin Monoclonal Antibody(4B11) was diluted at 1:200(4°C.overnight). 2. Sodium citrate pH 6.0 was used for antibody retrieval(>98°C.20min). 3.Secondary antibody was diluted at 1:200(room temperature. 30min). Negative control was used by secondary antibody only.
